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Effect of monoterpene citral on the expression of detoxification enzymes in HepaRG cells
Švábová, Tereza ; Boušová, Iva (advisor) ; Szotáková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Tereza Švábová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Consultant: Mgr. Michaela Šadibolová Title of diploma thesis: Effect of monoterpene citral on the expression of detoxification enzymes in HepaRG cells Citral is an acyclic monoterpene, which is mainly produced by higher plants. It is one of the many secondary metabolites, which serve the plant as detoxifying factors, morphoregulators and attractants for pollinators or defense substances. Besides other things, it has numerou positive effects such as antibacterial, anti-inflammatory or antitumor, but it may be toxic, especially for the liver, if used incorrectly. The main goal of this thesis was to find out the effect of citral on selected biotransformation and antioxidant enzymes in HepaRG cells. Differentiated HepaRG cells were incubated with 10 µM rifampicin, 10 µM β-naphthoflavone, 30 µM oltipraz, 10 µM citral, 30 µM citral and 100 µM citral for mRNA expression detection for 12 and 24 hours and for protein expression evaluation for 24 and 48 hours. At the mRNA level, rifampicin induced CYP3A4 at 12- and 24-h incubation, β-naphthoflavone induced CYP1A2 at 12- and 24-h incubation and aldo-ketoreductase (AKR1C) at 24-h incubation,...
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Effect of monoterpene citral on the expression of detoxification enzymes in HepaRG cells
Švábová, Tereza ; Boušová, Iva (advisor) ; Szotáková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Tereza Švábová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Consultant: Mgr. Michaela Šadibolová Title of diploma thesis: Effect of monoterpene citral on the expression of detoxification enzymes in HepaRG cells Citral is an acyclic monoterpene, which is mainly produced by higher plants. It is one of the many secondary metabolites, which serve the plant as detoxifying factors, morphoregulators and attractants for pollinators or defense substances. Besides other things, it has numerou positive effects such as antibacterial, anti-inflammatory or antitumor, but it may be toxic, especially for the liver, if used incorrectly. The main goal of this thesis was to find out the effect of citral on selected biotransformation and antioxidant enzymes in HepaRG cells. Differentiated HepaRG cells were incubated with 10 µM rifampicin, 10 µM β-naphthoflavone, 30 µM oltipraz, 10 µM citral, 30 µM citral and 100 µM citral for mRNA expression detection for 12 and 24 hours and for protein expression evaluation for 24 and 48 hours. At the mRNA level, rifampicin induced CYP3A4 at 12- and 24-h incubation, β-naphthoflavone induced CYP1A2 at 12- and 24-h incubation and aldo-ketoreductase (AKR1C) at 24-h incubation,...
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Antibiotic resistance in Staphylococcus aureus
Švábová, Tereza ; Vejsová, Marcela (advisor) ; Najmanová, Iveta (referee)
Author: Tereza Švábová Title: Resistance to antibiotics in Staphylococcus aureus Bachelor thesis Charles University in Prague, Faculty of Pharmacy, Hradec Králové Field of study: Medical Laboratory Background: The bachelor thesis aims at dealing with Staphylococcus aureus, describing its basic properties, laboratory diagnostics, antigenic structure and the diseases what it causes. Describe types and mechanisms of resistence, antibiotic therapy including mechanisms of antibiotic action. More attention is paid to the strains of MRSA (Methicillin resistant Staphylococcus aureus), because this is one of the biggest problems associated with resistance. Main findings: In the period before to the discovery of antibiotics, invasive infections caused by S. aureus were mostly fatal. With the introduction of antibiotics into practice, the problem of resistance has multiplied. The occurrence of MRSA was the largest in 2009 at - 14.6% in the Czech Republic. In 2014, the occurrence of MRSA was 13%, and 2 years later, there was again a slight increase - 13.9% in 2016 (ECDC, 2018). The selection of antibiotic and the method of antibiotic treatment of infections depend on the clinical condition of the patient, the agent of the disease including its sensitivity, the underlying disease and the properties of the...
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