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Two dimensionalk separations in Sequential Injection Chromatography low pressure system
Válová, Olga ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Olga Válová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of diploma thesis: Two Dimensional Separation in the Low Pressure System of Sequential Injection Chromatography The subject of this work was to construct a new method for separation of two analytes, which are different in their chemical properties and lipophilicity. Newly described method named Two Dimensional Sequential Injection Chromatography (2D-SIC) for isocratic system was applied in this work for the analysis of pharmaceutical preparation Otobacid N, which contains active substances Dexamethasone and Cinchocaine, i.e. substances with different chemical properties. The separation was carried out in two dimensions of two columns. In the first dimension for separation of Dexamethasone was used column 25 x 4.6 mm C-18 monolithic column with mobile phase acetonitrile/water in ratio of 35/65, flow rate 15 µl/sec. In the second dimension for separation of Cinchocaine was used column 10 x 4.6 mm C-18 monolithic column with precolumn 5 x 4.6 mm C-18 with mobile phase acetonitrile/water in ratio 60/40, flow rate 15 µl/sec. Optimal wavelength at 240 nm was chosen by spectrophotometric detection. Relative standard deviation...
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Two dimensionalk separations in Sequential Injection Chromatography low pressure system
Válová, Olga ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Olga Válová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of diploma thesis: Two Dimensional Separation in the Low Pressure System of Sequential Injection Chromatography The subject of this work was to construct a new method for separation of two analytes, which are different in their chemical properties and lipophilicity. Newly described method named Two Dimensional Sequential Injection Chromatography (2D-SIC) for isocratic system was applied in this work for the analysis of pharmaceutical preparation Otobacid N, which contains active substances Dexamethasone and Cinchocaine, i.e. substances with different chemical properties. The separation was carried out in two dimensions of two columns. In the first dimension for separation of Dexamethasone was used column 25 x 4.6 mm C-18 monolithic column with mobile phase acetonitrile/water in ratio of 35/65, flow rate 15 µl/sec. In the second dimension for separation of Cinchocaine was used column 10 x 4.6 mm C-18 monolithic column with precolumn 5 x 4.6 mm C-18 with mobile phase acetonitrile/water in ratio 60/40, flow rate 15 µl/sec. Optimal wavelength at 240 nm was chosen by spectrophotometric detection. Relative standard deviation...
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Two dimensionalk separations in Sequential Injection Chromatography low pressure system
Válová, Olga ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Olga Válová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of diploma thesis: Two Dimensional Separation in the Low Pressure System of Sequential Injection Chromatography The subject of this work was to construct a new method for separation of two analytes, which are different in their chemical properties and lipophilicity. Newly described method named Two Dimensional Sequential Injection Chromatography (2D-SIC) for isocratic system was applied in this work for the analysis of pharmaceutical preparation Otobacid N, which contains active substances Dexamethasone and Cinchocaine, i.e. substances with different chemical properties. The separation was carried out in two dimensions of two columns. In the first dimension for separation of Dexamethasone was used column 25 x 4.6 mm C-18 monolithic column with mobile phase acetonitrile/water in ratio of 35/65, flow rate 15 µl/sec. In the second dimension for separation of Cinchocaine was used column 10 x 4.6 mm C-18 monolithic column with precolumn 5 x 4.6 mm C-18 with mobile phase acetonitrile/water in ratio 60/40, flow rate 15 µl/sec. Optimal wavelength at 240 nm was chosen by spectrophotometric detection. Relative standard deviation...
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Two dimensionalk separations in Sequential Injection Chromatography low pressure system
Válová, Olga ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Olga Válová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of diploma thesis: Two Dimensional Separation in the Low Pressure System of Sequential Injection Chromatography The subject of this work was to construct a new method for separation of two analytes, which are different in their chemical properties and lipophilicity. Newly described method named Two Dimensional Sequential Injection Chromatography (2D-SIC) for isocratic system was applied in this work for the analysis of pharmaceutical preparation Otobacid N, which contains active substances Dexamethasone and Cinchocaine, i.e. substances with different chemical properties. The separation was carried out in two dimensions of two columns. In the first dimension for separation of Dexamethasone was used column 25 x 4.6 mm C-18 monolithic column with mobile phase acetonitrile/water in ratio of 35/65, flow rate 15 µl/sec. In the second dimension for separation of Cinchocaine was used column 10 x 4.6 mm C-18 monolithic column with precolumn 5 x 4.6 mm C-18 with mobile phase acetonitrile/water in ratio 60/40, flow rate 15 µl/sec. Optimal wavelength at 240 nm was chosen by spectrophotometric detection. Relative standard deviation...
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