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Characterization of pectin by ultrasonic spectroscopy
Trtek, Jan ; Kalina, Michal (referee) ; Hurčíková, Andrea (advisor)
Pectin solutions of various concentration in water has been investigated using high resolution ultrasonic spectroscopy. Furhermore, the influence of solvent on the properties of solutions by dissolving pectin in 0.15 M aqueous solution of sodium chloride and buffer PBS at pH 7.4. For comparison the properties of the pectin solutions were compared with solutions of pectate. It was found that pectine changes characteristics in differenet solutions. These changes have been observed due to changes in the parameters of ultrasonic waves.
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Interaction of hyaluronan with surfactant CAE
Trtek, Jan ; Pekař, Miloslav (referee) ; Hurčíková, Andrea (advisor)
The diploma thesis is focused on the study of interactions between hyaluronan of various molecular weights with CAE surfactant. This surfactant does not have the exact composition and there is not known their molecular weight. One of the main parameters needed to describe the interactions between surfactant and hyaluronan, there is the determination of critical micellar concentration. The value of critical micellar concentration of CAE is not known yet. All measurements were made for solutions in aqueous solution and 0.15 M NaCl. The determination of the molecular weight of this surfactant was performed by the technique SEC-MALS-dRI. High resolution ultrasonic spectroscopy was chosen to determine the critical micellar concentration and tensiometry was chosen as a complementary method. The interactions of CAE surfactant with polysaccharide of hyaluronan were showed by high resolution ultrasonic spectroscopy and densitometry. Compressibility was calculated from ultrasonic velocity and density.
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Interaction of hyaluronan with surfactant CAE
Trtek, Jan ; Pekař, Miloslav (referee) ; Hurčíková, Andrea (advisor)
The diploma thesis is focused on the study of interactions between hyaluronan of various molecular weights with CAE surfactant. This surfactant does not have the exact composition and there is not known their molecular weight. One of the main parameters needed to describe the interactions between surfactant and hyaluronan, there is the determination of critical micellar concentration. The value of critical micellar concentration of CAE is not known yet. All measurements were made for solutions in aqueous solution and 0.15 M NaCl. The determination of the molecular weight of this surfactant was performed by the technique SEC-MALS-dRI. High resolution ultrasonic spectroscopy was chosen to determine the critical micellar concentration and tensiometry was chosen as a complementary method. The interactions of CAE surfactant with polysaccharide of hyaluronan were showed by high resolution ultrasonic spectroscopy and densitometry. Compressibility was calculated from ultrasonic velocity and density.
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Characterization of pectin by ultrasonic spectroscopy
Trtek, Jan ; Kalina, Michal (referee) ; Hurčíková, Andrea (advisor)
Pectin solutions of various concentration in water has been investigated using high resolution ultrasonic spectroscopy. Furhermore, the influence of solvent on the properties of solutions by dissolving pectin in 0.15 M aqueous solution of sodium chloride and buffer PBS at pH 7.4. For comparison the properties of the pectin solutions were compared with solutions of pectate. It was found that pectine changes characteristics in differenet solutions. These changes have been observed due to changes in the parameters of ultrasonic waves.
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