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Monitoring of the influence of yeasts on the chemical and sensory qualities of manufactured wine
Petrášová, Ludmila ; Vítová, Eva (referee) ; Vránová, Dana (advisor)
The thesis deals with monitoring of the yeast's impact to the chemical and sensorial characteristics of produced wine. The aim of experimental part was obtaining of the aroma-active substances in the fermenting must and wine Hibernal and wine Pinot Blanc. SPME-GC-MS methods were used for identify wines and must aroma. The same autochthonous yeasts were used for wine production. This yeast was isolated from surface of grapes in our laboratory. It was found that both wines have a similar aromatic profile. The next aim was the monitoring of chemical parameters of samples and their changes. For this measurement were used spectrophotometric methods and HPLC methods. The last goal of the thesis was the isolation and identification of yeasts from wine Pinot Blanc by PCR-RFLP. These yeasts were then compared with yeasts isolated from Hibernal must. Identification of yeasts were done by amplification 5,8S-ITS DNA stretches using primers ITS1 and ITS4. Restriction endonuclease HaeIII, HinfI, HhaI and TaqI. were used for restriction analysis then.
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Monitoring of the influence of yeasts on the chemical and sensory qualities of manufactured wine
Petrášová, Ludmila ; Vítová, Eva (referee) ; Vránová, Dana (advisor)
The thesis deals with monitoring of the yeast's impact to the chemical and sensorial characteristics of produced wine. The aim of experimental part was obtaining of the aroma-active substances in the fermenting must and wine Hibernal and wine Pinot Blanc. SPME-GC-MS methods were used for identify wines and must aroma. The same autochthonous yeasts were used for wine production. This yeast was isolated from surface of grapes in our laboratory. It was found that both wines have a similar aromatic profile. The next aim was the monitoring of chemical parameters of samples and their changes. For this measurement were used spectrophotometric methods and HPLC methods. The last goal of the thesis was the isolation and identification of yeasts from wine Pinot Blanc by PCR-RFLP. These yeasts were then compared with yeasts isolated from Hibernal must. Identification of yeasts were done by amplification 5,8S-ITS DNA stretches using primers ITS1 and ITS4. Restriction endonuclease HaeIII, HinfI, HhaI and TaqI. were used for restriction analysis then.
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The influence of microflora on the sensoric characteristic of the wine
Petrášová, Ludmila ; Vítová, Eva (referee) ; Vránová, Dana (advisor)
The bachelor thesis is focused on the influence of the microflora on the sensory characteristics of the wine. The theoretical part provides information of the botanical description of Grüner Veltliner grape wine varietal, as well as its chemical composition, the white wine processing technology, and the influence of yeast on the aroma profile of this varietal. The experimental part describes the method of determining the aroma-active compounds by gas chromatography in combination with solid phase micro-extraction (SPME-GC). The object of the study was to measure samples of four different cider varieties of Grüner Veltliner during the fermentation process, and to compare the aromatic compounds in the cider with the indigenous yeast and in the cider where spontaneous fermentation was used.
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