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Study of deproteinization of milk samples via salting-out induced phase separation and automation by Lab-In-Syringe
Machián, Daniel ; Horstkotte, Burkhard (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Daniel Machián Supervisor: M.Sc. Burkhard Horstkotte, Ph.D. Co-supervisor: PharmDr. Kateřina Fikarová, Ph.D. Diploma thesis title: Study of deproteinization of milk samples via salting-out induced phase separation and automation by Lab-In-Syringe A novel approach to sample deproteinization based on homogeneous liquid-liquid extraction with salting-out induced phase separation was explored. A methodology was developed, experimentally tested, and optimized that allows automated centrifuge-less deproteinization of milk samples using the flow-batch technique Lab-In-Syringe. As in other sample preparation methods, the aim was to eliminate the sample matrix and obtain an organic phase that could be submitted to analysis. The thesis includes a range of preliminary experiments performed offline, i.e. without the intended instrumentation, as well as optimization experiments performed online using a Lab-In-Syringe system. Salting out agents and three solvents were tested as well as phase ratios and operational parameters on the Lab-In-Syringe system, including system configuration. In the method optimized by the work reported in this thesis, milk proteins were found effectively denatured and...
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