|
|
Analysis of coronavirus by QRT-PCR and possible therapy by nanoliposomal carriers of recombinant antigens
Krchová, Lenka ; doc. RNDr. Milan Bartoš. Ph.D. (referee) ; Turánek, Jaroslav (advisor)
This thesis deals with the study of coronaviruses, their diagnostics and subsequently the possible construction of a recombinant vaccine based on liposomal carriers of recombinant antigens. The experimental part of the thesis touches upon two levels. First, the diagnosis of SARS-CoV-2 virus was measured and investigated by real-time PCR. Biological material of a large number of patients was worked with. Together with the basic determination of the presence of the virus, the stability of the viral particle over time, its resistance to temperature changes, the effect of dilution of the carrier medium on the Ct value and other sub-experiments were investigated. From all the metadata, the most useful variant for pathogen diagnosis is finally evaluated. The experimental part is then followed by a possible therapy with recombinant vaccines. This issue is very topical and moves the whole professional community. Liposomes have been prepared which serve as carriers of bioactive substances in recombinant vaccines. The liposomes were homogenized by extrusion to the desired size and variously surface modified. Antigen binding, stability over time and possible degradation were investigated. Liposomes were characterized by DLS, nanoflow cytometry and electron microscopy.
|
|
|
Symmetric group, its representation and applications in molecular and quantum chemistry
Krchová, Lenka ; Tomáš, Jiří (referee) ; Kureš, Miroslav (advisor)
The subject of this bachelor thesis is the study of the symmetric groups, their representation and application in molecular chemistry. At first, the particular terms from the algebra are defined, which are which are necessary to define the concept of a group. Many of them are suúpplemented by pictures for clarity and better understanding. Then, the algebraic structures, which are accompanied by clear schemes and concrete examples, are explained. Also, the symmetric groups are demonstrated on the example of the square and triangle. After that, the reader gets into the chapter about the representation of final groups where the structure of the work is similar. First, the relevant terms are defined and then the author focuses on Young's diagrams. These are meticulůously described, few examples are mentioned and so is their working procedure. The last part of the bachelor thesis is dedicated to the operators in quantum chemistry, their principles and functions for two and three particles. This too is accompanied by examples.
|
|
|
Analysis of coronavirus by QRT-PCR and possible therapy by nanoliposomal carriers of recombinant antigens
Krchová, Lenka ; doc. RNDr. Milan Bartoš. Ph.D. (referee) ; Turánek, Jaroslav (advisor)
This thesis deals with the study of coronaviruses, their diagnostics and subsequently the possible construction of a recombinant vaccine based on liposomal carriers of recombinant antigens. The experimental part of the thesis touches upon two levels. First, the diagnosis of SARS-CoV-2 virus was measured and investigated by real-time PCR. Biological material of a large number of patients was worked with. Together with the basic determination of the presence of the virus, the stability of the viral particle over time, its resistance to temperature changes, the effect of dilution of the carrier medium on the Ct value and other sub-experiments were investigated. From all the metadata, the most useful variant for pathogen diagnosis is finally evaluated. The experimental part is then followed by a possible therapy with recombinant vaccines. This issue is very topical and moves the whole professional community. Liposomes have been prepared which serve as carriers of bioactive substances in recombinant vaccines. The liposomes were homogenized by extrusion to the desired size and variously surface modified. Antigen binding, stability over time and possible degradation were investigated. Liposomes were characterized by DLS, nanoflow cytometry and electron microscopy.
|
|
|
Symmetric group, its representation and applications in molecular and quantum chemistry
Krchová, Lenka ; Tomáš, Jiří (referee) ; Kureš, Miroslav (advisor)
The subject of this bachelor thesis is the study of the symmetric groups, their representation and application in molecular chemistry. At first, the particular terms from the algebra are defined, which are which are necessary to define the concept of a group. Many of them are suúpplemented by pictures for clarity and better understanding. Then, the algebraic structures, which are accompanied by clear schemes and concrete examples, are explained. Also, the symmetric groups are demonstrated on the example of the square and triangle. After that, the reader gets into the chapter about the representation of final groups where the structure of the work is similar. First, the relevant terms are defined and then the author focuses on Young's diagrams. These are meticulůously described, few examples are mentioned and so is their working procedure. The last part of the bachelor thesis is dedicated to the operators in quantum chemistry, their principles and functions for two and three particles. This too is accompanied by examples.
|
guest ::
RSS feed.