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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Study of the materno-fetal microchimerism of the APC using MHCII/EGFP mouse model and clearing histological techniques
 Knížková, Karolina ; Černý, Jan (advisor) ; Schwarzer, Martin (referee)
Microchimerism arises from the exchange of cells between genetically distinct individuals. The coexistence of genetically distinct cell populations within a single organism has possible effects on health and functioning of individuals immune systems, but the exact mechanisms of action are often not yet known. With the development of microscopic technologies and software for data analysis, the possibilities of detection and phenotyping of these rare cell populations are expanding. My intention in this work is to find maternal microchimerism in embryonic tissues (E13) and intestines of breastfed pups using MHCII/EGFP knock-in mouse model. Several different technologies potentially suitable for the detection of maternal microchimeric cells in offspring tissues (light sheet fluorescent microscopy - LSFM, virtual slide microscopy and flow cytometry) were selected. Advanced analysis of the obtained samples from the light sheet microscopy using the creation of a neural network was used here. The presence of maternal microchimerism was not demonstrated by flow cytometry. Using LSFM, image data were obtained from intestinal samples of suckling pups, which were processed by the neural network method. Data analysis of embryos (E13) obtained by the same method did not allow data analysis due to high...
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Maternal-fetal cellular trafficking: clinical implications and consequences
 Knížková, Karolina ; Černý, Jan (advisor) ; Petr, Jaroslav (referee)
Microchimerism is the presence of small population of cells with a different genetic information within the organism, which can result from bidirectional transfer of the cells between the mother and fetus during pregnancy. It is very studied phenomenon whose biological role is not clear yet. The presence of fetal cells in mother's body is associated with both positive and negative effects on maternal health. Microchimerism plays a role in cancer or autoimmune disease and it is implicated in development of tolerance mechanisms during pregnancy. Microchimerism could be used in prenatal diagnostics for aneuploidies or in prediction of complications during pregnancy.
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1 KNÍŽKOVÁ, Kateřina
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