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Histone deacetylase inhibitors in plasma cell leukemia treatment: effect of the bone marrow microenvironment
Burianová, Ilona ; Stöckbauer, Petr (advisor) ; Pour, Luděk (referee) ; Benson, Veronika (referee)
Multiple myeloma and its aggressive variant, plasma cell leukemia, are still considered to be incurable diseases despite the progressive treatment approaches comprising novel drugs. This can be attributed to the presence of the bone marrow microenvironment which plays an important role in drug resistance of myeloma cells. Hematopoietic cell lines derived from hematologic malignancies are suitable models for the study of etiopathogenesis of these malignant diseases and for testing new potential drugs. Establishment of these cell lines is still considered to be coincidental and rare event. The first part of the thesis is focused on establishment and characterization of the cell line UHKT-944 derived from a patient with primary plasma cell leukemia, and on completion of characterization of the cell line UHKT-893 derived from a patient with multiple myeloma. Additional analysis of UHKT-893 cell line were performed including sequence analysis of IgVH gene rearrangements and cytogenetic analysis which contributed to more detailed characterization of this cell line. During cultivation of UHKT-944 cells, we monitored the cell growth and confirmed dependence on interleukin-6 (IL-6). Immunophenotype analysis revealed the presence of surface markers characteristic of malignant plasma cells. UHKT-944 cells...
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Histone deacetylase inhibitors in plasma cell leukemia treatment: effect of the bone marrow microenvironment
Burianová, Ilona ; Stöckbauer, Petr (advisor) ; Pour, Luděk (referee) ; Benson, Veronika (referee)
Multiple myeloma and its aggressive variant, plasma cell leukemia, are still considered to be incurable diseases despite the progressive treatment approaches comprising novel drugs. This can be attributed to the presence of the bone marrow microenvironment which plays an important role in drug resistance of myeloma cells. Hematopoietic cell lines derived from hematologic malignancies are suitable models for the study of etiopathogenesis of these malignant diseases and for testing new potential drugs. Establishment of these cell lines is still considered to be coincidental and rare event. The first part of the thesis is focused on establishment and characterization of the cell line UHKT-944 derived from a patient with primary plasma cell leukemia, and on completion of characterization of the cell line UHKT-893 derived from a patient with multiple myeloma. Additional analysis of UHKT-893 cell line were performed including sequence analysis of IgVH gene rearrangements and cytogenetic analysis which contributed to more detailed characterization of this cell line. During cultivation of UHKT-944 cells, we monitored the cell growth and confirmed dependence on interleukin-6 (IL-6). Immunophenotype analysis revealed the presence of surface markers characteristic of malignant plasma cells. UHKT-944 cells...
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Rozdíly v účinnosti a fytotoxicitě vybraných insekticidů pro ošetření orchidejí
Burianová, Ilona
The Bachelor thesis "The differences in the efficiency and the phytotoxicity of selected insecticides for treating orchids" deals with the orchid pests and various kind of protecting measures against them. Efficiency of the individual preparation and plant reactions to treatment are verify too. Pests cause important deterioration of quality. Knowledge of pests and pathogens biology, their harmfulness economic importance is prerequisite for effective protection against them.
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