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Study of the effect of albendazole in the environment on the antioxidant system of red clover (Trifolium pratense)
Navrátilová, Gabriela ; Szotáková, Barbora (advisor) ; Langhansová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Gabriela Mikešková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: Study of the effect of albendazole in the environment on the antioxidant system of red clover (Trifolium pratense) Today, anthelmintics are among the essential drugs for the therapy and prophylaxis of infectious diseases caused by parasitic worms. However, these substances may be further released into the environment by the excrements of treated animals and affect non-target organisms, such as plants, which have the ability to absorb and accumulate various substances. In this study, we focused on the effect of the often used anthelmintic albendazole on the antioxidant system of red clover (Trifolium pratense), which is commonly found in meadows and pastures. The effect of low concentrations of albendazole available in the environment on the antioxidant enzymes catalase, superoxide dismutase, ascorbate peroxidase, peroxidase, glutathione peroxidase, glutathione reductase and glutathione S-transferase was studied. In addition to antioxidant enzymes, we also monitored the concentration of plant photosynthetic pigments chlorophyll a, b and carotenoids. The results were evaluated in control and...
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Regulation of morphogenic processes in potato - the role of sugar metabolism
Ševčíková, Hana ; Lipavská, Helena (advisor) ; Navrátil, Oldřich (referee) ; Langhansová, Lenka (referee)
Potato (Solanum tuberosum) tuberization is a complex, strictly regulated morphogenic process. Since potato is one of the most important crops in the world, understanding the regulation of this process is gaining in importance not only from a theoretical but also from a practical point of view. This work focuses on the role of sugars, which can serve not only as an energy and building material sources, but also as an important signal regulating many developmental processes including the tuberization. As a primary experimental material potato cv. Lada (WT) and its spontaneously tuberizing (ST) mutant was used. Since the previous results with ST plants suggested altered carbohydrate partitioning between plant organs and significant difference in total carbohydrate contents between ST plants and the WT (Fischer et al. 2008), in the first manuscript presented, the aim was to examine in detail the sugar metabolism of ST plants grown in vitro mixotrophically (MT) and photoautotrophically (PA). We observed changes in soluble carbohydrate allocation and starch deposition, favouring basal stem part in ST. Even thought, gibberellins (GAs) are considered to be the main tuberization inhibitors, ST potato plants had surprisingly high GAs levels. The determination of tuber-inducing marker gene expressions...
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Study of the effect of albendazole in the environment on the antioxidant system of red clover (Trifolium pratense)
Mikešková, Gabriela ; Szotáková, Barbora (advisor) ; Langhansová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Gabriela Mikešková Supervisor: prof. Ing. Barbora Szotáková, Ph.D. Title of diploma thesis: Study of the effect of albendazole in the environment on the antioxidant system of red clover (Trifolium pratense) Today, anthelmintics are among the essential drugs for the therapy and prophylaxis of infectious diseases caused by parasitic worms. However, these substances may be further released into the environment by the excrements of treated animals and affect non-target organisms, such as plants, which have the ability to absorb and accumulate various substances. In this study, we focused on the effect of the often used anthelmintic albendazole on the antioxidant system of red clover (Trifolium pratense), which is commonly found in meadows and pastures. The effect of low concentrations of albendazole available in the environment on the antioxidant enzymes catalase, superoxide dismutase, ascorbate peroxidase, peroxidase, glutathione peroxidase, glutathione reductase and glutathione S-transferase was studied. In addition to antioxidant enzymes, we also monitored the concentration of plant photosynthetic pigments chlorophyll a, b and carotenoids. The results were evaluated in control and...
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Regulation of morphogenic processes in potato - the role of sugar metabolism
Ševčíková, Hana ; Lipavská, Helena (advisor) ; Navrátil, Oldřich (referee) ; Langhansová, Lenka (referee)
Potato (Solanum tuberosum) tuberization is a complex, strictly regulated morphogenic process. Since potato is one of the most important crops in the world, understanding the regulation of this process is gaining in importance not only from a theoretical but also from a practical point of view. This work focuses on the role of sugars, which can serve not only as an energy and building material sources, but also as an important signal regulating many developmental processes including the tuberization. As a primary experimental material potato cv. Lada (WT) and its spontaneously tuberizing (ST) mutant was used. Since the previous results with ST plants suggested altered carbohydrate partitioning between plant organs and significant difference in total carbohydrate contents between ST plants and the WT (Fischer et al. 2008), in the first manuscript presented, the aim was to examine in detail the sugar metabolism of ST plants grown in vitro mixotrophically (MT) and photoautotrophically (PA). We observed changes in soluble carbohydrate allocation and starch deposition, favouring basal stem part in ST. Even thought, gibberellins (GAs) are considered to be the main tuberization inhibitors, ST potato plants had surprisingly high GAs levels. The determination of tuber-inducing marker gene expressions...
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