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Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis systém
Zouharová, Monika ; Horstkotte Šrámková, Ivana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Monika Zouharová Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Title: Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis system In this diploma thesis, we tested the use of various nanofibers as sorbents for solid phase extraction of neonicotinoids connected with sequential injection analysis system. Seven types of nanofibers were tested, specifically polyamide (PA6), polycaprolactone (PCL), polyimide (PID) and polydopamine coated polyamide (PA6)coated, polycaprolactone (PCL)coated, polyimide (PID)coated and a combination of polyimide and polycaprolactone (PID/PCL)coated. These nanofibers were produced by electrospinning. The studied nanofibers were placed in the 3D printed holder, which was connected into sequential injection analysis system. UV spectrophotometry was used as a detection method. The substances to analyse were neonicotinoid insectisides. The retention of these analytes was examinated with all seven types of nanofibers. Retention of the analytes on the tested nanofibers depending on their chemical structures and physical-chemical properties was studied in these measurements. The most experiments were performed with polydopamine coated...
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Development and optimization of a method for the determination of iodine in various matrices
Kušnír, Patrik ; Horstkotte Šrámková, Ivana (advisor) ; Chocholouš, Petr (referee)
Department: Department of Analytical Chemistry Candidate: Patrik Kušnír Supervisor: PharmDr. Ivana Šrámková, Ph.D. Tutor: doc. PharmDr. Hana Sklenářová, Ph.D. Title of diploma thesis: Development and optimization of a method for the determination of iodine in various matrices Iodine is an important trace element in our nutrition. Approximately two billions people in the world has insufficient iodine intake in nutrition. Defficiency in iodine causes insufficient production of thyroidal hormones, which is connected with various diseases such as goitre. That is why iodine is added to basic foodstuffs as table salt and other sources are important too, for example mineral water. It is important to monitor content of iodine to early discovery of disease risk connected with thyroidal gland and its diagnostics. The goal of this diploma thesis was to develop simple method with using of simple instrumental equipment to determine iodine in different samples. One part of development of our method was optimalization of conditions for iodine analysis in trace quantity and determination of iodine in selected samples. In-Syringe analysis, one of flow techniques, was used as a technique for accomplishing the task. This method was optimized for determination of iodine in sample. After optimalization this method was...
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Comparison of manual and dynamic extractions of selected transition metals from solid samples
Kucharčíková, Vendula ; Horstkotte, Burkhard (advisor) ; Horstkotte Šrámková, Ivana (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Vendula Kucharčíková Supervisor: Burkhard Horstkotte, Ph.D. Consultant: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of thesis: Comparison of manual and dynamic extractions of selected transition metals from solid samples The analysis of trace metals in soil is a subject of study in many laboratories. Due to large industrial areas soil can be highly contaminated. This work focused on two easy methods - manual extraction and single flow extraction with the same extractant -acetic acid. Two methods, two concentrations of acetic acid (0.11 M and 0.43 M), and two samples were used to evaluate, which conditions were more effective. As a detection device inductively coupled plasma - optical emission spectrometry was selected and two different wavelengths were used to prevent interferences. Manual extraction was more time-consuming and less reproducible than the dynamic extraction. Higher concentration of acetic acid extracted a larger amount of metals and the extraction showed higher reproducibility of the results. In both soils the contaminations with Cu outreached the permitted level for this metal (according to the requirement of the government of the Balearic Islands) and are potentially...
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Evaluation of antioxidant activity of natural compounds
Kunovská, Klára ; Sklenářová, Hana (advisor) ; Horstkotte Šrámková, Ivana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Klára Kunovská Supervisor: doc. PharmDr. Hana Sklenářová, PhD. Consultant: doc. RNDr. Miroslav Polášek, CSc. Title of Diploma Thesis: Evaluation of antioxidant activity of natural compounds The aim of this thesis was to evaluate antioxidant activity of natural compounds by chemiluminescence detection in flow system based on sequential injection analysis (SIA). The advantages of this system are high sensitivity, simplicity and high repeatability of individual analyses. The antioxidant activity was evaluated like dependence of chemiluminiscent signal on the concentration of tested antioxidant and then index Q was derived. Luminol was used as chemiluminiscent reagent, which showed chemiluminiscent emission in alkaline enviroment during oxidation of hydrogen peroxide together with a catalyst. As the catalyst, solution of potassium ferricyanide was used and whole measurement was conducted during working voltage of 435 mV. Chemiluminescent signal was monitored for a period of 60 seconds. For each tested substance, 3 cycles of measurement were conducted. The results of testing were evaluated using the area and height of the chemiluminescent signal and compared with the experiment without the tested...
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Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis systém
Zouharová, Monika ; Horstkotte Šrámková, Ivana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Monika Zouharová Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Title: Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis system In this diploma thesis, we tested the use of various nanofibers as sorbents for solid phase extraction of neonicotinoids connected with sequential injection analysis system. Seven types of nanofibers were tested, specifically polyamide (PA6), polycaprolactone (PCL), polyimide (PID) and polydopamine coated polyamide (PA6)coated, polycaprolactone (PCL)coated, polyimide (PID)coated and a combination of polyimide and polycaprolactone (PID/PCL)coated. These nanofibers were produced by electrospinning. The studied nanofibers were placed in the 3D printed holder, which was connected into sequential injection analysis system. UV spectrophotometry was used as a detection method. The substances to analyse were neonicotinoid insectisides. The retention of these analytes was examinated with all seven types of nanofibers. Retention of the analytes on the tested nanofibers depending on their chemical structures and physical-chemical properties was studied in these measurements. The most experiments were performed with polydopamine coated...
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Application of non-separation flow methods in pharmaceutical analysis
Šrámková, Ivana ; Sklenářová, Hana (advisor) ; Cabal, Jiří (referee) ; Miro, Manuel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Ivana Šrámková Supervisor: Assoc. Prof. Hana Sklenářová, Ph.D. Supervisor specialist: Burkhard Horstkotte, Ph.D. Title of the Dissertation Thesis: Application of non-separation flow techniques in pharmaceutical analysis Flow techniques are a branch of instrumental techniques in chemical analysis based on the handling of the sample in a tubing manifold. Since the introduction of what is recognized nowadays as modern flow techniques in the early seventies, this analytical approach gained popularity among scientist thanks to their main principles. Controlled dispersion and precise timing due to the programming enable full automation of a variety of analytical procedures. Flow methods were employed in a multiple different applications: simple sample measurement, chemical reactions, kinetic studies, sample pretreatment, separation, and others in an automated way. They are especially valued for automation, decrease of chemicals consumption and thus lower cost and waste production, and shortening of the analysis time. This dissertation presents new contributions to the field of flow techniques. It is divided into a theoretical part and an experimental part, the latter one listing five...
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Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis systém
Zouharová, Monika ; Horstkotte Šrámková, Ivana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Monika Zouharová Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Title: Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis system In this diploma thesis, we tested the use of various nanofibers as sorbents for solid phase extraction of neonicotinoids connected with sequential injection analysis system. Seven types of nanofibers were tested, specifically polyamide (PA6), polycaprolactone (PCL), polyimide (PID) and polydopamine coated polyamide (PA6)coated, polycaprolactone (PCL)coated, polyimide (PID)coated and a combination of polyimide and polycaprolactone (PID/PCL)coated. These nanofibers were produced by electrospinning. The studied nanofibers were placed in the 3D printed holder, which was connected into sequential injection analysis system. UV spectrophotometry was used as a detection method. The substances to analyse were neonicotinoid insectisides. The retention of these analytes was examinated with all seven types of nanofibers. Retention of the analytes on the tested nanofibers depending on their chemical structures and physical-chemical properties was studied in these measurements. The most experiments were performed with polydopamine coated...
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Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis systém
Zouharová, Monika ; Horstkotte Šrámková, Ivana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Monika Zouharová Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Title: Optimization of neonicotinoids extraction using nanofibers in sequential injection analysis system In this diploma thesis, we tested the use of various nanofibers as sorbents for solid phase extraction of neonicotinoids connected with sequential injection analysis system. Seven types of nanofibers were tested, specifically polyamide (PA6), polycaprolactone (PCL), polyimide (PID) and polydopamine coated polyamide (PA6)coated, polycaprolactone (PCL)coated, polyimide (PID)coated and a combination of polyimide and polycaprolactone (PID/PCL)coated. These nanofibers were produced by electrospinning. The studied nanofibers were placed in the 3D printed holder, which was connected into sequential injection analysis system. UV spectrophotometry was used as a detection method. The substances to analyse were neonicotinoid insectisides. The retention of these analytes was examinated with all seven types of nanofibers. Retention of the analytes on the tested nanofibers depending on their chemical structures and physical-chemical properties was studied in these measurements. The most experiments were performed with polydopamine coated...
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Study of nanofibers as sorbents for solid phase extraction using sequential injection analysis.
Štefčáková, Natália ; Horstkotte Šrámková, Ivana (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Natália Štefčáková Supervisor: PharmDr. Ivana Horstkotte Šrámková, Ph.D. Title: Study of nanofibers as sorbent for solid phase extraction using sequential injection analysis Various types of nanofibres were studied in the sequential injection analysis (SIA) system, where nanofibers were used as potential sorbents for solid phase extraction. Six types of nanofibers were tested in this thesis- polycaprolactone/polyvinylidene fluoride (PCL/PVDF), polyamide (PA 6), polystyrene (PS), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN) and polyethylene (PE). These nanofibers were produced by the electrospinning method. Substances with different physico-chemical properties were selected: bisphenol A and antibiotics oxytetracycline, amoxicillin, sulfadiazine and sulfathiazole. The nanofibers were placed in a 3D printed holder plugged into the SIA system. UV spectrophotometry was used as a detection technique. In this diploma thesis, parameters such as retention of substances on a given type of nanofibres, capacity of nanofibres and optimization of parameters for extraction of bisphenol A (sample volume, type, volume, concentration of eluent) were monitored and the 3D holder was tested as an...
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