National Repository of Grey Literature 31 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
The issue of urgent diagnostics of tick-borne encephalitis
In my bachelor thesis I dealt with urgent diagnostics of tick-borne encephalitis in the hospital of Havlíčkův Brod. The practical part was carried out in the immunology laboratory in the Joint Laboratories Unit in the hospital of Havlíčkův Brod, where the ChorusTrio device was purchased at the end of 2018. This device accelerated the diagnosis of samples from patients with suspected tick-borne encephalitis. Unfortunately, this device is only suitable for screening sera and is therefore mainly used to control antibodies before or after vaccination. In 2019 a new device, the ReaScan rapid test, was purchased and used for statim diagnostics. It is a simple and fast immunochromatographic test that allows us to confirm or refute the diagnosis of tick-borne encephalitis within 30 minutes. In the theoretical part I elaborated professional literature about tick-borne encephalitis, tick-borne encephalitis virus, its diagnostics and other related topics. In the practical part I dealt with methods whose principles are described in the theoretical part, in the chapter Laboratory diagnostics of tick-borne encephalitis in the hospital of Havlíčkův Brod, and whose working procedures are described in the chapter Methodology. From the obtained data and from the data from the LIS (Laboratory Information System) I compiled statistics, in which I evaluated the rate of return of the results from the laboratory examination. The aim of my bachelor thesis was to evaluate the existing statim diagnostics of tick-borne encephalitis with special emphasis on TAT (time of return of the examination results) and to find possible ways how to improve the diagnostics.
Interakce viru klíšťové encefalitidy s cytoskeletem hostitelských buněk
This thesis is focused on the role of host cytoskeleton, primarily microtubules and microfilaments, during tick-borne encephalitis virus infection in human neuroblastoma cell line SK-N-SH and tick cell line IRE/CTVM19. The importance of cytoskeletal integrity and dynamics to the viral replication cycle were examined using specific chemical inhibitors showing the virus utilizes studied structures in both cell lines. Immunofluorescence microscopy revealed structural changes in the actin cytoskeleton during late infection in SK-N-SH cells. Moreover, differences in expression of cytoskeleton-associated genes in both cell lines were compared. Several genes with up-regulated expression in SK-N-SH cells were identified during late infection.
Tick-borne encephalitis in Liberec Region during 2001-2016.
The presented thesis is focused on a comprehensive evaluation of situation related to the Tick-borne encephalitis on the territory of the Liberec Region in years 2001 2016. One of the main aims was to process the results of a demographic analysis of the Tick-borne encephalitis cases. These cases have been reported for 16 years within the so-called Tick-borne encephalitis surveillance to the system called EPIDAT by the Regional public health authority of the Liberec Region. Thank these analyzed data it was possible to create maps which illustrate particular focuses of the Tick-borne encephalitis in the Liberec Region and which graphically draw attention to locations threatened with infection. The KAP questionnaire study was held in a chosen Tick-borne encephalitis focus in order to improve a prevention of the Tick-borne encephalitis in the Region. This study was focused on knowledge, attitudes, opinions and practice of locals related to an occurrence of ticks and also of the Tick-borne encephalitis transmitted by them. Together with data gathering was held the distribution of the informative leaflets and also edification in this issue by a discussion about respondent´s questions. The collected data were processed by a descriptive method and also were detailed to a statistics testing. Due to an amount of analyzed information, this testing did not bring any statistic important results in most of the questions. The contribution of this thesis is especially the processed data of the Tick-borne encephalitis for those mentioned 16 years. These data could be useful for example for arguments of the public health authorities in case of discussion related to the Tick-borne encephalitis issue. The questionnaire study pointed out a problem which could cause a failure of people prevention. This problem means less of interest in health issues from their side. The question is how to learn population to perceive the risk of this disease when the half of the respondents do not feel threatened by the Tick-borne encephalitis? This fact is not certainly a problem only in the prevention of infectious diseases.
Potenciální antivirotické účinky derivátů přírodních látek proti viru klíšťové encefalitidy
VOKURKA, Radomír
This study was focused on potential antiviral effects of adamantane and its derivatives. Using flow cytometer was determined the most suitable concentration of adamantane and its derivatives which was not harmful for cells. The antiviral effect was measured on glioblastoma cells which were infected with tick-borne encephalitis virus. Virus concentration was determined using plaque assay.
Measurement of signalling pathway activation in mouse macrophage line IC-21 and primery dendritic cells after infection with tick-borne encephalitis virus.
Kožantová, Jana ; Lieskovská, Jaroslava (advisor) ; Drbal, Karel (referee)
Tick-borne encephalitis is a serious disease of the central nervous system. It is caused by tick-borne encephalitis virus, which is transmitted by ticks. The Czech Republic is one of the countries with the highest prevalence of this disease. Tick-borne encephalitis virus is able to replicate in several cell types. In this work we focused on macrophage line IC-21 and dendritic cells, because these cells are the first, which encounter the virus and support its spreading in the host at early stage of infection. So far there is not known any specific receptor for virus entry into cells or which signaling pathways activates. Therefore, we decided to investigate the activation of selected signaling pathways after infection with tick-borne encephalitis virus and influence of tick saliva on this activation. We employed methods of dual luciferase reporter assay, immunosandwich assay and western blot. The obtained results showed that in virus infected IC-21 cells are activated phosphatidyl-inositol pathway, NF-κB pathway, signaling molecule Erk1/2 and others. Testing of tick saliva effect revealed significantly decreased activity of NF-κB, AP-1 and CREB.
Tick-borne encephalitis vaccination and awareness of the disease risk factors in selected age group in South Bohemian Region
The thesis is devoted to the issue of vaccination problematics and awareness of tick-borne encephalitis in a selected population group in South Bohemia. For the needs of research, the selected group was composed of students of secondary schools and grammar schools in the South Bohemian Region in the age group from 13 to 19 years old. The objectives of this work was to analyze the incidence of tick-borne encephalitis in the Czech Republic and the South Bohemian Region between 2000 and 2015, to compare the vaccination in the Czech Republic with the other European countries, to find the vaccination of a selected age group in the South Bohemian Region and to analyze the level of awareness of the risks of tick-borne encephalitis by the polling method and the attitude of the age group to vaccination against this disease. To achieve the objectives was used a secondary analysis of the data from the EpiDat system, where the incidence of the disease was detected in the period in the South Bohemian Region and the Czech Republic. The questionnaire survey was used to obtain awareness on observed disease and attitude of the study group towards vaccination against tick-borne encephalitis. The conclusions of the questionnaire survey were obtained on the basis of statistical processing in the MS Excel spreadsheet. For my significant findings from my research, I consider 80% of the vaccination in the monitored group. Information on tick-borne encephalitis in most cases did not get the respondents from a doctor, but from family members, from the internet or from television. However, information on vaccination is given to respondents to a greater extent. Contributions by insurance companies do not motivate more than half of respondents to inoculation. Research has also shown, that respondents have insufficient information about the amount of the contribution and whether their insurance company provide the contribution at all. In view of these circumstances, I consider the increased promotion of this vaccination by insurance companies to be very desirable.
Diagnostics of Tick-borne Encephalitis: Present State and Future
Hustedová, Anna ; Španielová, Hana (advisor) ; Saláková, Martina (referee)
Tick-borne encephalitis is one of the most severe tick-borne neuroinfections in Europe and Asia. This thesis is focused on its source, Tick-borne encephalitis virus (TBEV). Diagnostics of TBEV is usually based on detection of specific anti-TBEV antibodies in patient's serum by using enzyme-linked immunosorbent assay. However antibodies that develop during the infection often cross-react with other flavivirus specific antibodies. As an antigen for ELISA tests the formalin inactivated virus is usually used, its production is restricted to laboratories with biosafety level 3. This thesis is seeking for possibilities of production of specific antigen for serological diagnostic of TBEV using heterologous expression systems. As the new antigens, parts of glycoprotein E and subviral particles were used. Production of antigen in the formo of subviral particles seems to be a good alternative to inactivated virus.
Students' knowledge of upper primary school about diseases transmitted by ticks
Michálková, Pavla ; Hanušová, Jaroslava (advisor) ; Thorovská, Alena (referee)
The main research problem concerns the characteristics of tick-borne disease and determine the quantity and quality of information that students get in health education and biology of these diseases. The theoretical part deals with the history, etiology, clinical features, diagnosis, treatment and prevention of tick-borne encephalitis and Lyme disease. It also discusses the general problems of tick and other diseases that ticks carry and cause. Also is mentioned the inclusion of tick-borne diseases in the educational program. The practical part is devoted to the awareness of upper primary school students and changes in their knowledge about the issue during their elementary education. The aim of this thesis is to determine how the students are informed about diseases transmitted by ticks, especially about Lyme disease and tick-borne encephalitis. This method is used questionnaire, which includes questions regarding this issue. Although the results of the research show a certain degree of knowledge of students at upper primary school, especially in some classes is unsatisfactory. Based on research, there is provided a recommendation for pedagogical practices. KEYWORDS: tick, tick-borne encephalitis, Lyme disease, knowledge, disease, upper primary school
Tick-Borne Diseases - Knowledge of High School Students
Vlček, Karel ; Pavlasová, Lenka (advisor) ; Ehler, Edvard (referee)
Tick-borne diseases are caused by a group of pathogenic microorganisms which are transmitted between animal and human population by vector which is most frequently tick. Evaluation of danger and perils of these diseases is important due to prevention and monitoring of current situation development. The most common tick-borne diseases in the Czech Republic are borreliosis, tick-borne encephalitis and ehrlichiosis. Rarely can we encounter bartonellosis, babesiosis, rickettsiosis and tularemia. All these diseases can have serious consequences and in critical cases they can result in death of the infected person. One of the basic and the most important of preventive measures which lower the risk of the infection by any tick-borne disease is prevention of tick encounter and eventually vaccination. Due to continual global warming we can expect that in near future ticks will spread even to locations which have been so far not suitable for their development. We can expect that we will even more frequently encounter tick-borne diseases - including diseases which were formerly not found in our territory or were very rare. As a part of health education it will be needed more to get known basic information of tick-borne diseases and their prevention. Different educational centres are an ideal place for...

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