National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
Dengue vaccines
Holšteinová, Aneta ; Šroller, Vojtěch (advisor) ; Poláková, Ingrid (referee)
Since the middle of the 20th century the number of people infected by the dengue virus (DENV) has been constantly increasing. Specific antiviral drugs are not available, so the objective for eliminating transmission has become the construction of an effective and safe vaccine with an emphasis on inducing a balanced immune reaction against all of the DENV serotypes. Momenteraly, the only licenced vaccine is CYD-TDV (Chimeric Yellow Fever- Dengue, Live-Attenuated, Tetravalent Dengue Vaccine). This vaccine uses the structure of the effective and safe vaccine against the yellow fever virus (YFV) from which surface protein sequences are substituted for corresponding DENV sequences. While preclinical studies displayed promising results, complications have arised during clinical studies, thus creating limiting criteria for their use. For this reason the search for new vaccines that could ensure better safety from DENV for a wider range of people and replace CYD-TDV is ongoing. In the III. phase of the clinical studies there are two live-attenuated vaccines (TetraVax-DV and DENVax). The subunite vaccine (V180), DNA vaccine (TVDV) or inactivated vaccine (TDENV PIV) was forwarded into the I. phase. The combination of several vaccine schedules is also being used (tetravalent live-attenuated...
Interakce viru klíšťové encefalitidy s cytoskeletem hostitelských buněk
This thesis is focused on the role of host cytoskeleton, primarily microtubules and microfilaments, during tick-borne encephalitis virus infection in human neuroblastoma cell line SK-N-SH and tick cell line IRE/CTVM19. The importance of cytoskeletal integrity and dynamics to the viral replication cycle were examined using specific chemical inhibitors showing the virus utilizes studied structures in both cell lines. Immunofluorescence microscopy revealed structural changes in the actin cytoskeleton during late infection in SK-N-SH cells. Moreover, differences in expression of cytoskeleton-associated genes in both cell lines were compared. Several genes with up-regulated expression in SK-N-SH cells were identified during late infection.
Preparation of the expression vectors for the production of fusion protein NS2-NS3 of Dengue and Zika viruses
Novotný, Pavel ; Konvalinka, Jan (advisor) ; Vaněk, Ondřej (referee)
in English Zika and Dengue viruses, which are part of Flaviridae family, are responsible for fever diseases. Infection can be asymptomatic or with symptoms of light virosis, however, it could be also life threatening. In the case of Dengue, it could progress to haemorrhagic fever. Infection by Zika is also dangerous for pregnant women because of enhanced risk of microcephaly in new-borns. These viruses are transferred by mosquitoes from Aedes family and are part of arbovirus group. Vaccination is a good tool for control of these diseases, but development of virostatics are also in progress. Inhibitors of viral proteases are important clinically approved virostatics against HIV and HCV. It is not clear, if Zika of Dengue proteases can become drug targets, however these proteases are also interesting for basic research. Flavivirus protease is located in viral polyprotein synthesized by host cells as a part of NS3 domain. This enzyme needs activation domain NS2B; mechanism of this interaction is not fully understood. On top of the protease activity, NS3 domain includes helicase, NTPase and RNA 5'-triphosphatase activity. It is not known why these enzymes coexist as one protein domain. To address these unanswered questions, it is important to prepare expression systems for kinetic, structure and...
The serological diagnostics of the tick-borne encefalitis in the University Hospital in Pilsen. The comparison of the tick-borne encefalitis occurance between the year 2011 and 2016.
The tick-borne encefalitis is an inflammatory virus illness which is transmitted to humans by infected ticks of germs Ixodes ricinus. These ticks have the virus in the saliva. RNA virus of the Flaviviridae section is the etiological agent. The virus belongs to the arboxiruses. The vaccination is the best preventive protection against this illness. This bachelor work is dedicated to the problems of tick-borne encefalitis in the Pilsen region. Two groups of patients, in the years 2011 and 2016, have been used to compare the results. The information about patients with tick-borne encefalitis in the year 2011 were taken from the laboratory information systém Open Lims in the virology sector of Institute of Microbiology of the University Hospital in Pilsen. I diagnosed the patients with confirmed tick-borne encefalitis by the method of Enzyme immunosorbent assay (ELISA) in the year 2016 and the antibodies were detected. ELISA is the routine diagnostic method which detects the tick-borne encefalitis antibodies in particular classes. Both groups of patients were evaluated according to the same criteria. The trend of occurence of tick-borne encefalitis has been surveyed with focusing on months, age cathegory and sex of the patients in the years 2011 and 2016. The detected data have been compared. The results of identified data show that this illness has the direct link to seasonal occurrence. The patients are mostly older adult people and there are more male patients than female. The results of both groups with serologicaly proven tick-borne encefalitis agree with the results in the EPIDAT systém in the years 2011 and 2016.

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