National Repository of Grey Literature 80 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Changes in Expression of Membrane Molecules CD200R, CD95, CD95L, and Soluble CD200R Regulating inflammatory Responses in Patients Undergoing Cardiac Surgery
Holmannová, Drahomíra ; Krejsek, Jan (advisor) ; Brát, Radim (referee) ; Turánek, Jaroslav (referee)
Cardiac surgery is known to initiate a complex physiological response with the immune system activation (SIRS), neurohormonal response, metabolic changes, coagulopathies etc. SIRS is triggered by tissue injury, myocardial ischemia, reperfusion, use of anaesthesia, cardioplegia, extracorporeal circuit etc. Excessive immune system activation is associated with progression of SIRS, life-threatening multi-organ dysfunction (MOD), and increased morbidity/mortality in the postoperative period. The immune system response is regulated and terminated by both cellular and humoral regulatory and inhibitory mechanisms including changes in expression of in our study monitored molecules: CD200/CD200R, sCD200R and CD95/CD95L. Methods: The study included the measurement the expression of CD95, CD95L, CD200R, and sCD200R molecules in granulocyte and monocyte populations in blood samples of 30 patients who underwent heart surgery using CPB. Samples collected before surgery, after surgery, and in the postoperative period (1st , 3rd , 7th day) were analysed by flow cytometry and sCD200R by ELISA. Results: We discovered a significant increase in the percentage of granulocytes expressing inhibitory molecule CD200R (from 5% to 17.8%) instantly after surgery. It might be presumed that these cells are less susceptible to...
Art group 12/15 Better Late than Never and its position in the Czech art after year 1989
Mayerová, Miroslava ; Bláha, Jaroslav (advisor) ; Čech, Viktor (referee)
Miroslava Mayerová Abstract Annotation Mayerová, Miroslava: Art group 12/15 and its position in the Czech art after year 1989. [Diploma thesis] Prague, 2019. Charles University in Prague, Faculty of education, Art education department. Title of the Diploma's Thesis: Art group 12/15 and its position in the Czech art after year 1989 ABSTRACT: The aim of the thesis is to describe and analyze the position of the Art Group 12/15 Better Late than never at Czech art scene after the year 1989, with an emphasis on generational changes and postmodern turn. In didactic part, the intention was to establish the awareness of the art group in the minds of students and, based on interdisciplinary ties, to repeat and broaden their knowledge of contexts. The theoretical part of the thesis is based on historical, art-historical and social science literature. To illustrate the time, atmosphere and position of Group 12/15 as precisely as possible, semi-structured and unstructured interviews with the group members themselves were used as a methodology. The diploma thesis shows that Group 12/15 and its generation are the bridge between the 2nd avant-garde, postmodern and contemporary art, maintaining the continuity of the Czech art scene. The didactic and practical part points out the importance of intergenerational discussion...
MEDIATRE OF COMMUNICATION IN CONTEMPORARY PAINTING
Kopecká, Marie ; Pfeiffer, Jan (advisor) ; Kornatovský, Jiří (referee)
The content of this bachelor thesis is a comprehensive insight into the problematics of communication in art with the aim of application of specific theoretical principles into artistic experience with emphasis on the aesthetic education. Theoretical part of this thesis consists of two parts. First one is focusing on the historical development of the role of art in society, perception of art and starting points, which led artists to specific steps. The second part is focusing on the present. Here I mention the theoretical approaches to the issue of perception of art and visual communication, focus on what role the viewer plays in the process of communication and I analyze communication strategies of selected Czech contemporary artists. In the practical part I am dealing with approaches in my own art work, which is specific application of conclusions mentioned in the theoretical part. In this part I am focusing more on the process of my own work, on choices of particular themes, its specific rendition and following reflection with the aim of verification of particular effects of each medium, which should be integral part of every work in art. The didactic part contains theoretical starting points, which led to making didactic series and my own designed didactic series for the first degree of...
Dance by image. (A dance movement as a theme in art and art education)
Ehlenová, Veronika ; Velíšek, Martin (advisor) ; Pfeiffer, Jan (referee)
The thesis focuses on the fusion of theoretical knowledge of visual art and dance art. The practical use of this knowledge has resulted in the realization of presented dance choreographies which are inspired by non-traditional artistic forms such as performance or happening, and are using their methods. The key point of the whole thesis is the movement, which is seen as a crucial part of the dance and visual arts, as well as a primary essence of the human existence. My work also shows how the theme of movement can be used when working with children. In various etudes with children, it includes various art techniques (drawing, painting, spatial creation). The practical part focuses on the expression of human emotions through the specific movements of the body, in the form of dance choreographies. It also uses new technologies, where photographs and video footage serve as an output. At the same time, the thesis shows the process of collaboration with other artists and necessary communication with them, which represents one of the crucial parts of the origin of an art work and one important chapter of this work as well. Taking account of specifics of both attitudes the aim of my thesis was to connect the fundamental components of visual art and dance which has together created a new perspective and...
Expression and purification of recombinant proteins of human Aichi virus and other kobuviruses
Ludvík, Tomáš ; Bouřa, Evžen (advisor) ; Petrvalská, Olívia (referee)
Viral 2Apro plays many important functions in the life cycle of virus. It is protease that is involved in the cleavage of the viral polyprotein in the VP1/2A region. The structure of protein contains active site, thanks to this the protein is able to bind various substrates in the host cell, which is essential for viral infection. With the help of 2Apro , the virus can bypass the host immune response and stop the production of key host proteins. Therefore, 2Apro is suitable target for antiviral intervention. In this work were prepared two recombinant proteins from Aichi virus and Coxsackievirus B3. These are viruses of the family Picornaviridae, which belong among the human pathogens that in some cases cause serious diseases. Aichi virus is the cause of gastroenteritis and Coxsackievirus B3 in the worst cases causes dilated cardiomyopathy. Cardiomyopathy leads to poor heart function due to it is enlargement and can lead to heart failure. (In Czech) Key words: recombinant proteins, 2Apro , Aichi virus, Coxsackievirus B3
Effect of tyrosine kinase inhibitor cabozantinib and cytotoxic alkaloid ellipticine on expression and activity of cytochromes P450 1A1, 1A2 and 1B1
Měkotová, Barbora ; Dračínská, Helena (advisor) ; Jeřábek, Petr (referee)
In recent years, tyrosine kinase inhibitors have been more and more used for the targeted cancer therapy, due to their ability to disrupt intracellular signalling pathways associated with the development of tumours. Cabozantinib is the tyrosine kinase inhibitor which has been approved for the treatment of thyroid cancer and it is also effective against several other types of cancer. However, multiple drugs combination is often used in anticancer therapy, which may result in their cytochrome P450-mediated interactions. Although this may affect the therapeutic effect of the drugs and cause adverse effects on the organism, very little is known about the effect of cabozantinib on biotransformation enzymes. Therefore, the effect of cabozantinib not only alone but also in combination with the known cytostatic ellipticine on the expression and the activity of cytochromes P450 1A1, 1A2 and 1B1 in rat liver and kidney in vivo was studied in this work. The gene expression was determined by quantitative PCR, the amount of protein was studied by Western blotting and consecutive immunodetection. The enzyme activity was studied using specific marker reactions, 7-ethoxyresorufin O-deethylation for CYP1A1, 7-methoxyresorufin O-demethylation for CYP1A2 and 17β-estradiol 4-hydroxylation for CYP1B1. Our results...
Integration site distribution of expressed proviruses
Miklík, Dalibor ; Hejnar, Jiří (advisor) ; Kejnovský, Eduard (referee) ; Indik, Stanislav (referee)
To establish efficient expression of their genes, retroviruses integrate proviral copies into the genomes of the cells they have infected. Epigenetic events, however, silence expression of the integrated proviruses. This silencing protects host cells from harmful viral spread, but also creates a reservoir of latent proviruses that subsequently hinders the cure of retroviral (e.g., HIV-1) infections. Furthermore, the silencing of retrovirus-derived integrative vectors complicates their application in transgenesis and gene therapy. The goal of this thesis is to describe the interaction between retroviral expression and host (epi)genomic environment at the site of proviral integration. To pursue the goal, we sought to define the (epi)genomic environment of the proviruses, which expression is not affected by the epigenetic silencing. Diverse retroviral vectors derived from avian sarcoma and leukosis virus (ASLV), murine leukemia virus (MLV), and human immunodeficiency virus type 1 (HIV-1) were used as model retroviral systems, and expression stability of the vectors in human cell lines was examined. In order to identify the features unique to integration sites of the active proviruses, we sorted the cells positive for the proviral expression, identified their proviral integration sites, and compared them to...
Expression and purification of recombinant Sericin-2 and Salivary gland secretion 3
VU, Trang Thanh
Recombinant proteins derived from Bombyx mori Ser-2 gene and Drosophila melanogaster Sgs3 gene were expressed in bacterial expression systems, purified and analyzed using different molecular biology techniques. The pure proteins were tested for cell adhesive properties by coating hydrophobic surfaces with the proteins and monitoring the growth of adherent cells in vitro. By showing adequate expression and resultant yield, as well as the ability to aid cell adhesion, the adhesive proteins present great potential for future biomaterials.
Expression of selected membrane transporters in placentas of pregnant women diagnosed for preterm rupture of membranes
Michalská, Martina ; Čečková, Martina (advisor) ; Jirkovský, Eduard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Martina Michalská Supervisor: doc. PharmDr. Martina Čečková, Ph.D. Title of diploma thesis: Expression of selected membrane transporters in placentas of pregnant women diagnosed for preterm rupture of membranes Placenta is a key organ for pregnancy maintenance. One of its main functions is transport of compounds between mother and her fetus. The transplacental penetration is ensured due to membrane transporters that are present in the apical or basal side of trophoblast. Their expression level is affected by many physiological and pathological factors, among others it can be influenced by infection and inflamatory reaction. Inflammation is also one of the risk factors of preterm deliveries and it can be therefore assumed that these pathological states are accompanied by changes in expression of placental transporters. This study was performed using 51 placentas obtained from Faculty hospital in Hradec Králové from women who underwent preterm delivery and on 15 placentas delivered in term. The study employed quantitative RT-PCR approach. The mRNA expression of membrane transporters ABCB1, ABCG2, OATP1A2, OATP1B3, OATP2A1, OATP2B1, OATP3A1, OATP4A1 was assessed and the results were compared to...
Body as a live image
Popovičová, Gabriela ; Čech, Viktor (advisor) ; Hůla, Zdenek (referee)
The master's thesis named Body as a live image is dealing with the topic of the body in the art education. The focus of the theoretical part of the work is to describe basic theoretical starts points and evolution of the body art, including descriptions of characteristics of typologists streams in wide contexts, connect the topic of the body with the art education describe it in the pedagogy context as well. The focus of the empirical part of the thesis is to describe authors own work with the given topic, describe possible use of the topic of the body in the art education and to describe the realization of the theme line in the creative art class. The theoretical base in master's thesis is described in general contexts with the evolution of the body including art education and pedagogy. Author's works with the topic of the body create an open compilation. Work includes thematic art assignation for practical pedagogy, following by the self-reflection of the teacher and suggestions for the improvement. The master's thesis focus on the created typology, categorized into streams and the author's artwork with the topic of the body. There is an emphasis on the line of creative assignation that is followed by the created typology of the streams. The part of the realization is a self-reflection of the...

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