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Fungal genetics, genome evolution and use of flow cytometry in study of DNA
Würtherlová, Tereza ; Kolařík, Miroslav (advisor) ; Kostovčík, Martin (referee)
The fungal genome is a dynamically changing structure. By its remodelling, the organism can respond to the environmental changes and develop itself. The genome expansion is often accompanied by transition to parasitic or mutualistic way of life. The genome expansion can be caused by the multiplication of some chromosomes (aneuploidisation), the whole genome duplication (polyploidisation) or the spreading of repetitive sequences. The impact of genome size to the ecology and life strategy of the organisms is more and more studied in recent years. In mycology, nevertheless, it escapes sufficient attention. The flow cytometry (FCM) is a modern and progressively developing method that enables to determine the genome size and estimate CG/AT base ratio. The combination of FCM with knowledge of the fungal ecology and forces that form the genome enable to discover a general trends of the evolutionary processes. My study summarises knowledge about the processes leading to changes in the size/structure of the fungal genome, the correlations with genome size and presents the principle of flow cytometry and its application in mycology.
Fungal genetics, genome evolution and use of flow cytometry in study of DNA
Würtherlová, Tereza ; Kostovčík, Martin (referee) ; Kolařík, Miroslav (advisor)
The fungal genome is a dynamically changing structure. By its remodelling, the organism can respond to the environmental changes and develop itself. The genome expansion is often accompanied by transition to parasitic or mutualistic way of life. The genome expansion can be caused by the multiplication of some chromosomes (aneuploidisation), the whole genome duplication (polyploidisation) or the spreading of repetitive sequences. The impact of genome size to the ecology and life strategy of the organisms is more and more studied in recent years. In mycology, nevertheless, it escapes sufficient attention. The flow cytometry (FCM) is a modern and progressively developing method that enables to determine the genome size and estimate CG/AT base ratio. The combination of FCM with knowledge of the fungal ecology and forces that form the genome enable to discover a general trends of the evolutionary processes. My study summarises knowledge about the processes leading to changes in the size/structure of the fungal genome, the correlations with genome size and presents the principle of flow cytometry and its application in mycology.