Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.01 vteřin. 
Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina ; Bačáková, Lucie ; Pařízek, Martin ; Vandrovcová, Marta ; Musílková, Jana ; Lisá, Věra ; Gabor, R. ; Marvan, J.
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants.
Computational study of cellular assembly on hydrophobic/hydrophilic micro-patterns
Ukraintsev, Egor ; Brož, A. ; Kalbáčová, M.H. ; Kromka, Alexander ; Rezek, Bohuslav
We develop simple onedimensional stochastic model of cell behavior on chemically patterned surfaces that is based on three key parameters: speed of cell movement (motility) across substrate, probability of cell adhesion to substrate, and probability of cell division on substrate when adhered on substrate. Amount of adhered cells on hydrophobic and hydrophilic regions is calculated as function of time (number of cycles up to 2000). The model is correlated with in-vitro data obtained within 48 h in real time. We show that this simple stochastic model with the three parameters (where cell motility is the most important one) can describe with high accuracy the experimental data and thereby explain the observed preferential cell assembly on hydrophilic/hydrophobic micro-patterns (up to 200 um width).

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