National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Methodology of detection Penicillium expansum, Monilia frutigena, Botrytis cinerea and Neofabraea alba using multiplex PCR
Sumíková, Taťána ; Gabrielová, Ludmila ; Žabka, Martin
Multiplex PCR for rapid and reliable detection of species causing rotting apples: Penicillium expansum, Monilia fructigena, Botrytis cinerea and Neofabraea alba is described here. Reaction is optimized for using in a common molecular laboratory. Described method allows both detectino of the pathogens from pure mycological cultrures, and their detection directly from the rot apple tissues. Cultivation of the micromycethes on cellophane surface is also described. References for mycological determination literature are listed, and can be helpful for determination in case of negative results of PCR.
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