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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Drobnička – vodní rostlina jako nový modelový organismus pro studium signálních drah a biotechnologie
 Matysková, Tereza
Wolffia spp. are aquatic plants with extremely reduced morphology and rapid reproduction cycle with the shortest recorded doubling time of under 30 hours. These smallest known flowering plants are thus excellent for biotechnology and studying molecular mechanisms underlying growth and development. The theoretical part of this thesis reviews Wolffia spp. and discusses prospective future applications. The experimental part is dedicated to the characterization of W. australiana proteome, employing two complementary strategies for proteome fractionation using an off-line C18 peptides fractionation and an online ion mobility-based fractionation technique. That part is followed by a proteome analysis of W. australiana grown in nutrient-rich media. Finally, results of proteome and metabolome profiling of W. australiana in response to cadmium ions are presented.
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Proteome dynamics during seed germination
 Habánová, Hana
Seed germination is a crucial phase of the plant life cycle. Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination are far from being resolved. Most of the early events of germination are mediated by molecules stored in seeds during the seed development and thus we employed proteomic analyses to elucidate the mechanism behind this process. This thesis - Proteome dynamics during seed germination - reviews present-day knowledge of this topic. In the experimental part, the results of three related research projects are summarized. First, the effects of a novel class of growth regulators were analyzed in the barley germination assay and the optimal concentrations were determined. The methodology for an improved analysis of barley seed proteins was developed and the optimized targeted analysis will allows the detection of over 4,000 proteins. Finally, Arabidopsis thaliana seed proteome was analyzed and the results indicate that (i) an alternative MS/MS data processing significantly improves the detection limits and (ii) the proteasome-ubiquitin system plays a crucial role in adjusting the seed proteome dynamics during the germination progress.
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