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Optimization of the optical setup for chemiluminescence detection of natural products
Laho, Tomáš ; Dian, Juraj (advisor) ; Hraníček, Jakub (referee)
The content of the bachelor thesis was the implementation of a new luminescent apparatus and the study of chemiluminescence of luminol and beer samples of suitable age. The chemiluminescence of luminol was used in this work to determine its basic characteristics (spectrum, intensity of chemiluminescence, and its dependence on the composition of the reaction mixture) using a fiber spectrometer and, subsequently, verify the functionality of the assembled apparatus. This work aimed to realize an apparatus suitable for the chemiluminescence measurement of natural substances with potential use in the food industry. Luminol made it possible to observe intense chemiluminescence, thanks to which it was possible to optimize the measured signal. After successful optimization, there was an attempt to detect the chemiluminescent response in samples of artificially aged beer and compare the results obtained with those already published in the professional literature. In the presented bachelor thesis, the characteristics of luminol chemiluminescence were measured using a fiber spectrometer. Based on these results, the parameters of the new luminescent apparatus (size of slots, voltage on the photomultiplier) and the maximum values of chemiluminescence intensity for the correct operation of the apparatus were...
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