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Study of bioactive compounds of fennel
Mierna, Jana ; Skoumalová, Petra (referee) ; Veselá, Mária (advisor)
The master´s thesis is focused on the study of biologically active compounds of fennel. The theoretical part describes basic characteristics of fennel and its chemical composition, further specifies plant´s biological active substances and antioxidants, also describes extraction techniques for obtaining these bioactive compounds, methods of their determination and verification of antimicrobial effect. In the experimental part were spectrophotometrically determined contents of polyphenols and flavonoids in the prepared extracts of fennel tea, whole and crush spice. On the basis of extraction curves, antioxidant activity and potential antimicrobial activity against the selected microorganisms Bacillus subtilis, Bacillus cereus, Serratia marcescens a Micrococcus luteus was determined in the samples with the highest values of the given bioactive substances. Among the all monitored extracts, the inhibitory effect was showed only by an aqueous extract of the fennel tea against the bacterial strain of Micrococcus luteus.
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Sea buckthorn – the source of natural compounds
Blašková, Daniela ; Vítová, Eva (referee) ; Veselá, Mária (advisor)
The main aim of the bachelor thesis was a research of common sea buckthorn, often denoted as "a plant of future". Botanic characteristics of the given bush, verification of biologically active substances and various concentration extract effects on selected kinds of microorganisms. In the theoretical part, the literature search is done, which discusses the characterization of the researched plant. The practical part deals with the application of selected methods of research, as follows: spectrophotometric determination of biologically active substances and microbiological verification of microbial activity on microorganisms, such as Bacillus subtilis, Serratia marcenscens and Micrococcus luteus. Considering the thesis results, it ensues that the given extracts do not show too great bacterial activity. Ex adverso, the extracts contain too big amount of determined active substances, in some cases.
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Study of bioactive compounds of fennel
Mierna, Jana ; Skoumalová, Petra (referee) ; Veselá, Mária (advisor)
The master´s thesis is focused on the study of biologically active compounds of fennel. The theoretical part describes basic characteristics of fennel and its chemical composition, further specifies plant´s biological active substances and antioxidants, also describes extraction techniques for obtaining these bioactive compounds, methods of their determination and verification of antimicrobial effect. In the experimental part were spectrophotometrically determined contents of polyphenols and flavonoids in the prepared extracts of fennel tea, whole and crush spice. On the basis of extraction curves, antioxidant activity and potential antimicrobial activity against the selected microorganisms Bacillus subtilis, Bacillus cereus, Serratia marcescens a Micrococcus luteus was determined in the samples with the highest values of the given bioactive substances. Among the all monitored extracts, the inhibitory effect was showed only by an aqueous extract of the fennel tea against the bacterial strain of Micrococcus luteus.
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|
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Sea buckthorn – the source of natural compounds
Blašková, Daniela ; Vítová, Eva (referee) ; Veselá, Mária (advisor)
The main aim of the bachelor thesis was a research of common sea buckthorn, often denoted as "a plant of future". Botanic characteristics of the given bush, verification of biologically active substances and various concentration extract effects on selected kinds of microorganisms. In the theoretical part, the literature search is done, which discusses the characterization of the researched plant. The practical part deals with the application of selected methods of research, as follows: spectrophotometric determination of biologically active substances and microbiological verification of microbial activity on microorganisms, such as Bacillus subtilis, Serratia marcenscens and Micrococcus luteus. Considering the thesis results, it ensues that the given extracts do not show too great bacterial activity. Ex adverso, the extracts contain too big amount of determined active substances, in some cases.
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