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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Molekulární detekce bakterií rodu \kur{Helicobacter} u laboratorních myší
 FEDOR, Tereza
This study investigates the molecular detection of Helicobacter bacteria in laboratory mice. Bacteria of the genus Helicobacter are known for their ability to infect the gastrointestinal tract of mammals. This research focuses on the development and optimization of methods for the sensitive detection of these bacteria from buccal swabs of mice. Molecular methods such as TaqMan real-time PCR, conventional PCR and cloning have been used. The results of this study may help to determine the prevalence of infected mice in research laboratories, which may be useful for further research in gastrointestinal health, and treatment of related diseases.
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Optimization of Real-Time PCR for detection of Klebsiella pneumoniae
 ELSTNEROVÁ, Šárka
The subject of this bachelor's thesis is the optimization of real-time PCR for the detection of the bacteria Klebsiella pneumoniae using primers from the publication Sun et al, 2010, for SYBR Green real-time PCR and end point PCR and primers designed by the supervisor of this thesis (Stehlíková, unpublished) for TaqMan real-time PCR. The selected primers were subsequently tested on pure culture samples of Klebsiella pneumoniae 4799 from the DSMZ collection and verified on 41 buccal swabs. Furthermore, assessment of specificity was accomplished using 26 bacterial DNA isolates. Assessment of sensitivity of PCR detection methods was accomplished depending on their routine use, assessment of their limits of detection and comparison.
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