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Role of Fibroblasts in Regulation of Wound Healing
Zavaďáková, Anna ; Vištejnová, Lucie (advisor) ; Buchtová, Marcela (referee) ; Krulová, Magdaléna (referee)
Dermal fibroblasts participate actively in the regulation of wound healing process. They migrate and proliferate during the proliferative phase into the wound site and respond to signals from damaged tissue by secretion of a various pro-inflammatory molecules (e.g. interleukins IL6 and IL8) and extracellular matrix components (e.g. collagen type I, hyaluronan, fibronectin). The disruption of the balance and timing of synthesis and degradation of these molecules can lead to the transition of normal to abnormal non-healing process. Several stress factors act simultaneously in this type of wounds - impaired nutrition supply, inflammation, bacterial contamination, oxidative stress etc. However, the latest in vitro research of dermal fibroblasts in wound healing apply most often only one stress factor. This thesis deals with the response of dermal fibroblasts to the wound conditions. For the first time, a comprehensive study of the functional response of the cells to the two key wound stress factors is described in 2D culture and 3D self-made collagen hydrogel culture. In the first part of the thesis response of dermal fibroblasts to the wound conditions applying two stress factors - low nutrition (2% FBS) and inflammation simulated by bacterial lipopolysaccharide (LPS) - is characterized. The...
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