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The effect of trans-nerolidol and valencene in ovarian cancer cells
Zajdlová, Martina ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martina Zajdlová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of trans-nerolidol and valencene in ovarian cancer cells Trans-nerolidol and valencene belong to sesquiterpenes, which present a large group of secondary plant metabolites. In practice, we can encounter them in the food or perfume industry. However, they are also used in traditional Chinese medicine. Recently, the biological activities of sesquiterpenes, which include e.g. anti-inflammatory or antineoplastic activities, were studied. Doxorubicin is one of the most important cytostatic drugs and various ways to improve its effectiveness and reduce side effects are being studied. One such usage is in combination therapy with natural substances, e.g. with sesquiterpenes. The aim of this study was to test antiproliferative effects of doxorubicin, trans-nerolidol and valencene on ovarian cancer cell lines SK-OV-3 and A2780. We also assayed the possible pro-oxidant potential of doxorubicin and terpenes by measuring the production of ROS. In addition, we evaluated the interaction between doxorubicin and terpenes by quantification of combination indexes. We also studied the possible effect of...
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Nanofibrous materials in bone tissue engineering
Zajdlová, Martina ; Bačáková, Lucie (advisor) ; Míčová, Petra (referee)
This thesis focuses on nanofibrous materials which are highly promising regarding they application in a modern interdisciplinary scientific field - tissue engineering. Through the years there have been developed various strategies for creating materials usable in tissue engineering. The earliest materials that were made did not allow any cell adhesion on their surfaces (so-called "bioinert" materials), whereas nowadays there is an effort to create hybrid bioartificial organs. Especially in bone tissue engineering do polymeric materials in the form of a nanofibrous network, such as polylactide or polycaprolactone with the addition of inorganic particles (for example nanocrystalic hydroxyapatite), show great potential. Such materials mimic the natural bone tissue and stimulate the adhesion, proliferation and differentiation of cells into desirable a cell type. In the experimental part of this thesis one of these promising nanomaterials was tested for its biocompatibility in vitro. Polylactide in the form of nanofibrous networks with 0, 5 and 15 % of nanocrystallic hydroxyapatite was provided by Elmarco s.r.o, Liberec. Human osteoblast-like cells MG 63 were cultivated on these materials for 1, 3 and 7 days. The results show the convenience of hydroxyapatite particles which stimulate the cells to the...
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The effect of trans-nerolidol and valencene in ovarian cancer cells
Zajdlová, Martina ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martina Zajdlová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of trans-nerolidol and valencene in ovarian cancer cells Trans-nerolidol and valencene belong to sesquiterpenes, which present a large group of secondary plant metabolites. In practice, we can encounter them in the food or perfume industry. However, they are also used in traditional Chinese medicine. Recently, the biological activities of sesquiterpenes, which include e.g. anti-inflammatory or antineoplastic activities, were studied. Doxorubicin is one of the most important cytostatic drugs and various ways to improve its effectiveness and reduce side effects are being studied. One such usage is in combination therapy with natural substances, e.g. with sesquiterpenes. The aim of this study was to test antiproliferative effects of doxorubicin, trans-nerolidol and valencene on ovarian cancer cell lines SK-OV-3 and A2780. We also assayed the possible pro-oxidant potential of doxorubicin and terpenes by measuring the production of ROS. In addition, we evaluated the interaction between doxorubicin and terpenes by quantification of combination indexes. We also studied the possible effect of...
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The effect of trans-nerolidol and valencene in ovarian cancer cells
Zajdlová, Martina ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martina Zajdlová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of trans-nerolidol and valencene in ovarian cancer cells Trans-nerolidol and valencene belong to sesquiterpenes, which present a large group of secondary plant metabolites. In practice, we can encounter them in the food or perfume industry. However, they are also used in traditional Chinese medicine. Recently, the biological activities of sesquiterpenes, which include e.g. anti-inflammatory or antineoplastic activities, were studied. Doxorubicin is one of the most important cytostatic drugs and various ways to improve its effectiveness and reduce side effects are being studied. One such usage is in combination therapy with natural substances, e.g. with sesquiterpenes. The aim of this study was to test antiproliferative effects of doxorubicin, trans-nerolidol and valencene on ovarian cancer cell lines SK-OV-3 and A2780. We also assayed the possible pro-oxidant potential of doxorubicin and terpenes by measuring the production of ROS. In addition, we evaluated the interaction between doxorubicin and terpenes by quantification of combination indexes. We also studied the possible effect of...
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Nanofibrous materials in bone tissue engineering
Zajdlová, Martina ; Bačáková, Lucie (advisor) ; Míčová, Petra (referee)
This thesis focuses on nanofibrous materials which are highly promising regarding they application in a modern interdisciplinary scientific field - tissue engineering. Through the years there have been developed various strategies for creating materials usable in tissue engineering. The earliest materials that were made did not allow any cell adhesion on their surfaces (so-called "bioinert" materials), whereas nowadays there is an effort to create hybrid bioartificial organs. Especially in bone tissue engineering do polymeric materials in the form of a nanofibrous network, such as polylactide or polycaprolactone with the addition of inorganic particles (for example nanocrystalic hydroxyapatite), show great potential. Such materials mimic the natural bone tissue and stimulate the adhesion, proliferation and differentiation of cells into desirable a cell type. In the experimental part of this thesis one of these promising nanomaterials was tested for its biocompatibility in vitro. Polylactide in the form of nanofibrous networks with 0, 5 and 15 % of nanocrystallic hydroxyapatite was provided by Elmarco s.r.o, Liberec. Human osteoblast-like cells MG 63 were cultivated on these materials for 1, 3 and 7 days. The results show the convenience of hydroxyapatite particles which stimulate the cells to the...
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