|
|
Study of influence of quantum dots to biological systmes and their components
Koudelková, Zuzana ; Zítka, Ondřej (referee) ; Provazník, Ivo (advisor)
The aim of this thesis is to summarize the available evidence about quantum dots and their effects on living systems. The text describes methods for the preparation of quantum dots with respect to their characteristics (size, fluorescence wavelength) and methods of quantum dots bio-functionalization of biomolecules. In living organisms is a large number of proteins, because these are considered as one of the main components of the interaction of organisms. Therefore, the work also provides basic information about proteins. Finally, there are described various methods by which the quantum dots may be characterized mainly by differential pulse voltammetry measurement zeta potential and fluorescence measurement. The main objective of this work is to propose models of different environments in which will be degradation of quantum dots with regard to the evaluation of acquired kinetic parameters for predicting the stability of individual quantum dots.
|
|
| |
|
| |
|
|
Methods for study of oxidative stress in oncology patients using flow injection analysis with electrochemical detection
Zítka, Ondřej ; Bořek Dohalská, Lucie (referee)
Thesis: Method for study of oxidative stress in oncology patients using flow injection analysis with electrochemical detection ABSTRACT An oxidative damage of an organism and its mechanisms belong to the main goals of interest in the area of biochemistry. The substances causing such type of damage, where oxygen radicals are mentioned mostly, affect an organism on the level of the most important biomolecules such as DNA. Due to this fact the transformation of nucleic bases in DNA chain to its analogues occurs and thus the functionality of genetic code may be changed leading to pathological processes. The aim of this thesis is to show the suitability of electrochemical methods for sensitive detection of products of oxidation of nucleic acids as a marker of oxidative damage. Using methods as ELISA, flow injection system and magnetic particles, the sensor for detection of 8-OHdG as a marker of oxidation of nucleic acids at oncological patients was designed and successfully applied. By using of developed method the samples of urine of fourteen patients suffering from prostate cancer were analyzed. We were able to divide the patients into three levels based on determined concentration of 8-OHdG. Whole method is not even sufficiently sensitive but it is suitable for purposes of application into the microfluidic...
|
|
|
Study of influence of quantum dots to biological systmes and their components
Koudelková, Zuzana ; Zítka, Ondřej (referee) ; Provazník, Ivo (advisor)
The aim of this thesis is to summarize the available evidence about quantum dots and their effects on living systems. The text describes methods for the preparation of quantum dots with respect to their characteristics (size, fluorescence wavelength) and methods of quantum dots bio-functionalization of biomolecules. In living organisms is a large number of proteins, because these are considered as one of the main components of the interaction of organisms. Therefore, the work also provides basic information about proteins. Finally, there are described various methods by which the quantum dots may be characterized mainly by differential pulse voltammetry measurement zeta potential and fluorescence measurement. The main objective of this work is to propose models of different environments in which will be degradation of quantum dots with regard to the evaluation of acquired kinetic parameters for predicting the stability of individual quantum dots.
|
guest ::
