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Determination of acetylsalicylic acid by sequential injection analysis with spectrophotometric detection
Vrtalová, Martina ; Hraníček, Jakub (advisor) ; Červený, Václav (referee)
This diploma thesis is focused on the determination of acetylsalicylic acid using two methods of flow analysis; Flow Injection Analysis (FIA) and Sequential Injection Analysis (SIA) with spectrophotometric detection. The principle of determination is based on the acetylsalicylic acid decomposition to give salicylic ion. This ion reacts subsequently with Fe3+ ion in acidic solution leading to colored chelating complex Fe(SAL)+ . The increase of absorbance of this complex in its absorption maximum was used for analytical determination. Sodium salicylate (sodium salt of acetylsalicylic acid) was used as a standard substance. In the first part of this work, experimental arrangement for flow injection analysis was setup. Continuously the optimization of experimental conditions of acetylsalicylic acid determination was performed to obtain the higher sensitivity. Under the optimal conditions the basic characteristics of determination was measured. In the second part of this work, the commercial experimental instrument FIA Lab 3500 was used for determination. Optimization of experimental conditions and basic characteristic of determination by sequential injection analysis was performed as well. Developed methods for determination of acetylsalicylic acid with selected technique were verified by analyzing...
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Influence of salicylic acid on naproxene and diclofenac phytoextraction.
Vrtalová, Martina ; Smrček, Stanislav (advisor) ; Břichnáčová Habartová, Věra (referee)
The influence of salicylic acid on the naproxene a diclofenac phytoextraction at different starting concentrations of tested substances has been investigated. The experiments were performed using in vitro sterile hydroponic cultivation of Helianthus annus, Pisum sativum, Brassica napus and Zea mays plants on the Murashige-Skoog cultivation medium supplemented with appropriate combinations of tested substances. The extraction of naproxene (15 or 17 mg/l) in the presence of salicylic acid (1 or 5 mg/l) was decreased at B. napus and P. sativum plants. In contrast to these results the phytoextraction by H. annuus plants was increased by the use of the same experimental conditions . At diclofenac/salicylic acid combinations and H. annuus plants the positive influence of salicylic acid in the concentration of 1 mg/l on diclofenac phytoextraction was found while the concentration of 5 mg/l diminished the phytoextraction efficiency. At other used plant species the extraction of diclofenac was concentration dependently decreased by salicylic acid. The obtained results clearly show that phytoextraction in multicomponent mixtures is complicated and in tested systems the salicylic acid moderately influenced phytoextraction of naproxene and diclofenac in dependence on the used plant species ad salicylic acid...
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Determination of acetylsalicylic acid by sequential injection analysis with spectrophotometric detection
Vrtalová, Martina ; Hraníček, Jakub (advisor) ; Červený, Václav (referee)
This diploma thesis is focused on the determination of acetylsalicylic acid using two methods of flow analysis; Flow Injection Analysis (FIA) and Sequential Injection Analysis (SIA) with spectrophotometric detection. The principle of determination is based on the acetylsalicylic acid decomposition to give salicylic ion. This ion reacts subsequently with Fe3+ ion in acidic solution leading to colored chelating complex Fe(SAL)+ . The increase of absorbance of this complex in its absorption maximum was used for analytical determination. Sodium salicylate (sodium salt of acetylsalicylic acid) was used as a standard substance. In the first part of this work, experimental arrangement for flow injection analysis was setup. Continuously the optimization of experimental conditions of acetylsalicylic acid determination was performed to obtain the higher sensitivity. Under the optimal conditions the basic characteristics of determination was measured. In the second part of this work, the commercial experimental instrument FIA Lab 3500 was used for determination. Optimization of experimental conditions and basic characteristic of determination by sequential injection analysis was performed as well. Developed methods for determination of acetylsalicylic acid with selected technique were verified by analyzing...
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Influence of salicylic acid on naproxene and diclofenac phytoextraction.
Vrtalová, Martina ; Smrček, Stanislav (advisor) ; Břichnáčová Habartová, Věra (referee)
The influence of salicylic acid on the naproxene a diclofenac phytoextraction at different starting concentrations of tested substances has been investigated. The experiments were performed using in vitro sterile hydroponic cultivation of Helianthus annus, Pisum sativum, Brassica napus and Zea mays plants on the Murashige-Skoog cultivation medium supplemented with appropriate combinations of tested substances. The extraction of naproxene (15 or 17 mg/l) in the presence of salicylic acid (1 or 5 mg/l) was decreased at B. napus and P. sativum plants. In contrast to these results the phytoextraction by H. annuus plants was increased by the use of the same experimental conditions . At diclofenac/salicylic acid combinations and H. annuus plants the positive influence of salicylic acid in the concentration of 1 mg/l on diclofenac phytoextraction was found while the concentration of 5 mg/l diminished the phytoextraction efficiency. At other used plant species the extraction of diclofenac was concentration dependently decreased by salicylic acid. The obtained results clearly show that phytoextraction in multicomponent mixtures is complicated and in tested systems the salicylic acid moderately influenced phytoextraction of naproxene and diclofenac in dependence on the used plant species ad salicylic acid...
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