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Progress of welding in shipbuilding
Urban, Václav ; Kubíček, Jaroslav (referee) ; Kovář, Petr (advisor)
This work deals with the methods of welding in shipbuilding, summarises the basic techniques used for welding of the SAW, MAG, MIG, TIG, laser, Laser-Hybrid. It describes the properties of welded material. Especially the materials HY80 and HY100. It follows from the filler materials. In particular materials Spoolarc 95 and 120. Particular emphasis is placed on the method of Laser-Hybrid, its properties and use in shipbuilding. Finally, describes the method of welding aluminium using FSW, preparation of welded surfaces and producting of sandwich panels.
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Incineration of Sludge Mixed with Alkali
Urban, Václav ; Jecha, David (referee) ; Houdková, Lucie (advisor)
Subject of the present Ing. thesis is problem of incineration of sewage sludge and influence of lime on production of editions. In work are described kinds of sewage sludge combustion and possibilities of edition cleaning. Work includes design and description of experimental combustion of sewage sludge mixtured with kalcite in fluidized bed reactor. The end of work delal with, evaluation of editions from combustion experiment. Evaluated components of bunt gases are: heavy metals, TZL, SO2, CO, CO2, HCl, HF, O2, NO, PAH, PCB, PCDD/F.
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The role of RECQ5 helicase in maintanance of genome stability
Urban, Václav ; Janščák, Pavel (advisor) ; Krejčí, Lumír (referee) ; Blažek, Dalibor (referee)
DNA replication is the most vulnerable process of DNA metabolism in proliferating cells and therefore it is tightly controlled and coordinated with processes that maintain genomic stability. Human RecQ helicases are among the most important factors involved in the maintenance of replication fork integrity, especially under conditions of replication stress. Collisions between replication and transcription machineries represent a significant source of genomic instability. RECQ5 DNA helicase binds to RNA-polymerase (RNAP) II during transcription elongation and suppresses transcription-associated genomic instability. Here we show that RECQ5 also associates with RNAPI and enforces the stability of ribosomal DNA arrays in cells exposed to replication stress. We demonstrate that RECQ5 associates with transcription complexes in DNA replication foci and counteracts replication fork stalling in RNAPI- and RNAPII-transcribed genes, suggesting that RECQ5 exerts its genome stabilizing effect by acting at sites of concomitant replication and transcription. Moreover, RECQ5- deficient cells accumulate RAD51 foci that are formed in a BRCA1-dependent manner at sites of interference between replication and transcription and likely represent unresolved replication intermediates. Importantly, BRCA1-dependent formation...
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The role of RECQ5 helicase in maintanance of genome stability
Urban, Václav ; Janščák, Pavel (advisor) ; Krejčí, Lumír (referee) ; Blažek, Dalibor (referee)
DNA replication is the most vulnerable process of DNA metabolism in proliferating cells and therefore it is tightly controlled and coordinated with processes that maintain genomic stability. Human RecQ helicases are among the most important factors involved in the maintenance of replication fork integrity, especially under conditions of replication stress. Collisions between replication and transcription machineries represent a significant source of genomic instability. RECQ5 DNA helicase binds to RNA-polymerase (RNAP) II during transcription elongation and suppresses transcription-associated genomic instability. Here we show that RECQ5 also associates with RNAPI and enforces the stability of ribosomal DNA arrays in cells exposed to replication stress. We demonstrate that RECQ5 associates with transcription complexes in DNA replication foci and counteracts replication fork stalling in RNAPI- and RNAPII-transcribed genes, suggesting that RECQ5 exerts its genome stabilizing effect by acting at sites of concomitant replication and transcription. Moreover, RECQ5- deficient cells accumulate RAD51 foci that are formed in a BRCA1-dependent manner at sites of interference between replication and transcription and likely represent unresolved replication intermediates. Importantly, BRCA1-dependent formation...
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Incineration of Sludge Mixed with Alkali
Urban, Václav ; Jecha, David (referee) ; Houdková, Lucie (advisor)
Subject of the present Ing. thesis is problem of incineration of sewage sludge and influence of lime on production of editions. In work are described kinds of sewage sludge combustion and possibilities of edition cleaning. Work includes design and description of experimental combustion of sewage sludge mixtured with kalcite in fluidized bed reactor. The end of work delal with, evaluation of editions from combustion experiment. Evaluated components of bunt gases are: heavy metals, TZL, SO2, CO, CO2, HCl, HF, O2, NO, PAH, PCB, PCDD/F.
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Progress of welding in shipbuilding
Urban, Václav ; Kubíček, Jaroslav (referee) ; Kovář, Petr (advisor)
This work deals with the methods of welding in shipbuilding, summarises the basic techniques used for welding of the SAW, MAG, MIG, TIG, laser, Laser-Hybrid. It describes the properties of welded material. Especially the materials HY80 and HY100. It follows from the filler materials. In particular materials Spoolarc 95 and 120. Particular emphasis is placed on the method of Laser-Hybrid, its properties and use in shipbuilding. Finally, describes the method of welding aluminium using FSW, preparation of welded surfaces and producting of sandwich panels.
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