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National Repository of Grey Literature

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The use of real time PCR method for evaluation of magnetic microspheres Trachtová, Š. ; Rittich, B. ; Horák, Daniel ; Španová, A. The real-time quantitative PCR (qPCR) was used for the evaluation of non-porous HEMA-based magnetic microspheres on the PCR course (PCR sensitivity). It was shown that 1-10 mu l microsphere suspensions (2 mg/ml) in PCR mixtures do not interfere in the PCR. It can be assumed that the magnetic nuclei were covered with polymer layer. Detailed record

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