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The influence of the dominant trees and grain substrate the composition of the microbial community studied by PLFA
Stachová, Sandra ; Frouz, Jan (advisor) ; Heděnec, Petr (referee)
The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...
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Quantitative analysis by X-Ray powder diffraction
Stachová, Sandra ; Havlíček, David (advisor) ; Vojtíšek, Pavel (referee)
Quantitative analysis methods using X-ray powder diffraction are still highly actual conception in the area of research of modern materials. The Reference Intensity Ratio (RIR) method is used here for quantitative analysis by X-ray powder diffraction of model samples in this bachelor thesis. The model samples are three mixtures of corundum, mullite and calcite. To get diffraction patterns of the mixtures, two different diffractometers are used and their outputs are then compared.
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The influence of the dominant trees and grain substrate the composition of the microbial community studied by PLFA
Stachová, Sandra ; Frouz, Jan (advisor) ; Heděnec, Petr (referee)
The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...
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Quantitative analysis by X-Ray powder diffraction
Stachová, Sandra ; Havlíček, David (advisor) ; Vojtíšek, Pavel (referee)
Quantitative analysis methods using X-ray powder diffraction are still highly actual conception in the area of research of modern materials. The Reference Intensity Ratio (RIR) method is used here for quantitative analysis by X-ray powder diffraction of model samples in this bachelor thesis. The model samples are three mixtures of corundum, mullite and calcite. To get diffraction patterns of the mixtures, two different diffractometers are used and their outputs are then compared.
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