|
|
The human skin barrier model containing stripped stratum corneum
Soukupová, Romana ; Pullmannová, Petra (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Author: Romana Soukupová Supervisor: Mgr. Petra Pullmanová, Ph.D. Title of diploma thesis: The human skin barrier model containing stripped stratum corneum The uppermost layer of the epidermis, the stratum corneum, acts as a protective skin barrier. It contains a lipid matrix, which is made up of ceramides, cholesterol, and free fatty acids. The composition and arrangement of these lipids are necessary for the proper function of the skin barrier. Corneocytes, which do not have nuclei, are distributed in the lipid matrix. They are surrounded by insoluble proteins with covalently bound lipids on their surface, creating the corneocyte lipid envelope. The aim of this diploma thesis was to prepare models of the human skin barrier using an isolated stratum corneum with a preserved corneocyte lipid envelope and without a preserved corneocyte lipid envelope, and then monitor and compare the lipid arrangements in these models. Obtaining the stratum corneum from the skin samples was necessary to prepare the models. Human skin lipids were extracted from isolated stratum corneum and part of this delipidated stratum corneum was saponified. Skin lipids were purificated by column chromatography, and their...
|
|
|
The human skin barrier model containing stripped stratum corneum
Soukupová, Romana ; Pullmannová, Petra (advisor) ; Kováčik, Andrej (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Author: Romana Soukupová Supervisor: Mgr. Petra Pullmanová, Ph.D. Title of diploma thesis: The human skin barrier model containing stripped stratum corneum The uppermost layer of the epidermis, the stratum corneum, acts as a protective skin barrier. It contains a lipid matrix, which is made up of ceramides, cholesterol, and free fatty acids. The composition and arrangement of these lipids are necessary for the proper function of the skin barrier. Corneocytes, which do not have nuclei, are distributed in the lipid matrix. They are surrounded by insoluble proteins with covalently bound lipids on their surface, creating the corneocyte lipid envelope. The aim of this diploma thesis was to prepare models of the human skin barrier using an isolated stratum corneum with a preserved corneocyte lipid envelope and without a preserved corneocyte lipid envelope, and then monitor and compare the lipid arrangements in these models. Obtaining the stratum corneum from the skin samples was necessary to prepare the models. Human skin lipids were extracted from isolated stratum corneum and part of this delipidated stratum corneum was saponified. Skin lipids were purificated by column chromatography, and their...
|
guest ::
RSS feed.