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Neuromarketing: emocionální apely v reklamách v odvětví cestovního ruchu
Sokolová, Simona
Krejčová, S. Neuromarketing: emotional appeals in tourism advertising. Diploma thesis. Brno: Mendel University in Brno, 2023. This diploma thesis is focused on the phenomenon of emotional appeals used in tourism advertisements from a neuromarketing perspective. These appeals are adventure, humor, calm, family, romance, fear, and disgust. The goal of the thesis was to provide recommendations for the development of marketing strategies in the tourism industry based on marketing research. The secondary goal was to investigate the brain response in selected EEG metrics to these appeals. In the theoretical part, topics related to marketing, neuromarketing, emotional appeals, and tourism were covered. In the practical part, research was conducted which consisted of using a combination of eye-tracking and EEG devices. The research was supplemented by a questionnaire survey. The measured data from the research was analysed using statistics and was supplemented with the results from the questionnaires. It was found out that there was no preference for the humor appeal over the other observed appeals. In particular, the preferred emotional appeal was the appeal of calm followed by the appeal of romance and adventure. Participants were most reluctant to the appeal of disgust.
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Characterization of human warfarin reductase
Sokolová, Simona ; Malátková, Petra (advisor) ; Skarka, Adam (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Simona Sokolová Supervisor: PharmDr. Petra Malátková, Ph.D. Title of diploma thesis: Characterization of human warfarin reductase Warfarin is widely used anticoagulant drug. Considering the narrow therapeutic window of warfarin, it is important to fully understand its metabolism in human body. Oxidative, reductive and conjugation reactions are involved in warfarin metabolism. However, the reductive metabolism of warfarin has not been studied in details until now. The reduced metabolite of warfarin, i.e. warfarin-alcohol, is produced by the conversion of the carbonyl group of the side chain. It is known that human liver cytosolic and microsomal fractions exhibit warfarin reductase activity but the specific enzymes catalysing the reduction of warfarin are not known yet. The aim of this study was to identify the enzyme(s) participating in reduction of warfarin and to describe enzyme kinetics. Human liver cytosolic and microsomal fractions and recombinant enzymes AKR1A1, AKR1B1, AKR1B10, AKR1C1, AKR1C2, AKR1C3, AKR1C4, CBR1 and CBR3 were incubated with warfarin at various concentrations. The produced warfarin-alcohol was quantified by UHPLC and the specific activities of enzymes and...
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Characterization of human warfarin reductase
Sokolová, Simona ; Malátková, Petra (advisor) ; Skarka, Adam (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Simona Sokolová Supervisor: PharmDr. Petra Malátková, Ph.D. Title of diploma thesis: Characterization of human warfarin reductase Warfarin is widely used anticoagulant drug. Considering the narrow therapeutic window of warfarin, it is important to fully understand its metabolism in human body. Oxidative, reductive and conjugation reactions are involved in warfarin metabolism. However, the reductive metabolism of warfarin has not been studied in details until now. The reduced metabolite of warfarin, i.e. warfarin-alcohol, is produced by the conversion of the carbonyl group of the side chain. It is known that human liver cytosolic and microsomal fractions exhibit warfarin reductase activity but the specific enzymes catalysing the reduction of warfarin are not known yet. The aim of this study was to identify the enzyme(s) participating in reduction of warfarin and to describe enzyme kinetics. Human liver cytosolic and microsomal fractions and recombinant enzymes AKR1A1, AKR1B1, AKR1B10, AKR1C1, AKR1C2, AKR1C3, AKR1C4, CBR1 and CBR3 were incubated with warfarin at various concentrations. The produced warfarin-alcohol was quantified by UHPLC and the specific activities of enzymes and...
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Characterization of human warfarin reductase
Sokolová, Simona ; Malátková, Petra (advisor) ; Skarka, Adam (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Simona Sokolová Supervisor: PharmDr. Petra Malátková, Ph.D. Title of diploma thesis: Characterization of human warfarin reductase Warfarin is widely used anticoagulant drug. Considering the narrow therapeutic window of warfarin, it is important to fully understand its metabolism in human body. Oxidative, reductive and conjugation reactions are involved in warfarin metabolism. However, the reductive metabolism of warfarin has not been studied in details until now. The reduced metabolite of warfarin, i.e. warfarin-alcohol, is produced by the conversion of the carbonyl group of the side chain. It is known that human liver cytosolic and microsomal fractions exhibit warfarin reductase activity but the specific enzymes catalysing the reduction of warfarin are not known yet. The aim of this study was to identify the enzyme(s) participating in reduction of warfarin and to describe enzyme kinetics. Human liver cytosolic and microsomal fractions and recombinant enzymes AKR1A1, AKR1B1, AKR1B10, AKR1C1, AKR1C2, AKR1C3, AKR1C4, CBR1 and CBR3 were incubated with warfarin at various concentrations. The produced warfarin-alcohol was quantified by UHPLC and the specific activities of enzymes and...
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Characterization of human warfarin reductase
Sokolová, Simona ; Malátková, Petra (advisor) ; Skarka, Adam (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Simona Sokolová Supervisor: PharmDr. Petra Malátková, Ph.D. Title of diploma thesis: Characterization of human warfarin reductase Warfarin is widely used anticoagulant drug. Considering the narrow therapeutic window of warfarin, it is important to fully understand its metabolism in human body. Oxidative, reductive and conjugation reactions are involved in warfarin metabolism. However, the reductive metabolism of warfarin has not been studied in details until now. The reduced metabolite of warfarin, i.e. warfarin-alcohol, is produced by the conversion of the carbonyl group of the side chain. It is known that human liver cytosolic and microsomal fractions exhibit warfarin reductase activity but the specific enzymes catalysing the reduction of warfarin are not known yet. The aim of this study was to identify the enzyme(s) participating in reduction of warfarin and to describe enzyme kinetics. Human liver cytosolic and microsomal fractions and recombinant enzymes AKR1A1, AKR1B1, AKR1B10, AKR1C1, AKR1C2, AKR1C3, AKR1C4, CBR1 and CBR3 were incubated with warfarin at various concentrations. The produced warfarin-alcohol was quantified by UHPLC and the specific activities of enzymes and...
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