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In vitro biodegradation of biopolyesters exposed to synthetic gastric juice
Sobková, Markéta ; Skoumalová, Petra (referee) ; Kovalčík, Adriána (advisor)
This bachelor’s thesis is focused on the study of in vitro biodegradation of biopolyesters exposed synthetic gastric juice and phosphate buffer saline with lipase for 81 days. The theoretical part summarises the necessary information about characteristics of biodegradable biopolyesters, deals with their possible applications in medicine. The experimental part deals with the biodegradation of prepared biopolyesters films. in this work, poly(lactic acid), amorphous poly(hydroxy alkanoates), poly(3-hydroxybutyrate), poly(butylene adipate terephthalate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), filament poly(lactic acid) and filament poly(lactic acid)/poly(hydroxybutyrate) were used for the experiment. The films were prepared by dissolving of the biopolyesters in hot chloroform. The dissolving agent was evaporated, and prepared films were dried. Prepared films had different properties depending on the used biopolyesters. Their appearance was formed transparent to milky cloudy. Also, trey differed in their plasticity. During biodegradation, the morphology changed, and the samples gained higher fragility. The effect of biodegradation was monitored by changes in 1) molecular weights (SEC-MALLS), 2) chemical structure (FTIR) and 3) weight loss changes (gravimetrically). The analysed biopolyesters were demonstrably susceptible to biodegradation in simulated body conditions. The degree of biodegradation depends on the type of biopolyester. The most significant weight loss was recorded for the sample of amorphous polyhydroxyalkanoate (PHA). The PHA´original weight was reduced about 84.28 ± 0.03 % after the 81 days of biodegradation in the phosphate-buffered saline with lipase.
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In vitro biodegradation of biopolyesters exposed to synthetic gastric juice
Sobková, Markéta ; Skoumalová, Petra (referee) ; Kovalčík, Adriána (advisor)
This bachelor’s thesis is focused on the study of in vitro biodegradation of biopolyesters exposed synthetic gastric juice and phosphate buffer saline with lipase for 81 days. The theoretical part summarises the necessary information about characteristics of biodegradable biopolyesters, deals with their possible applications in medicine. The experimental part deals with the biodegradation of prepared biopolyesters films. in this work, poly(lactic acid), amorphous poly(hydroxy alkanoates), poly(3-hydroxybutyrate), poly(butylene adipate terephthalate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), filament poly(lactic acid) and filament poly(lactic acid)/poly(hydroxybutyrate) were used for the experiment. The films were prepared by dissolving of the biopolyesters in hot chloroform. The dissolving agent was evaporated, and prepared films were dried. Prepared films had different properties depending on the used biopolyesters. Their appearance was formed transparent to milky cloudy. Also, trey differed in their plasticity. During biodegradation, the morphology changed, and the samples gained higher fragility. The effect of biodegradation was monitored by changes in 1) molecular weights (SEC-MALLS), 2) chemical structure (FTIR) and 3) weight loss changes (gravimetrically). The analysed biopolyesters were demonstrably susceptible to biodegradation in simulated body conditions. The degree of biodegradation depends on the type of biopolyester. The most significant weight loss was recorded for the sample of amorphous polyhydroxyalkanoate (PHA). The PHA´original weight was reduced about 84.28 ± 0.03 % after the 81 days of biodegradation in the phosphate-buffered saline with lipase.
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