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Creation and analysis of conditional knock-out CDK12 for the study of female reproductive ability
Sedmíková, Veronika ; Šušor, Andrej (advisor) ; Frolíková, Michaela (referee)
Oogenesis is a process with a complex interplay of changes at the genetic, cellular, and structural levels that should lead to the differentiation of female gametes. Strict regulation of these processes is required, as any disruption can lead to fertility problems or disabilities in the offspring. The aim of this work is to gain further insight into the processes that influence oocyte development. This work focuses on cyclin-dependent kinase 12 (CDK12), which belongs to the serine/threonine kinase family. It is known for its pleiotropic role in cellular processes such as transcription, translation, cell cycle progression, cell proliferation, DNA damage response and maintenance of genome stability. CDK12 forms an active complex with its binding partner Cyclin K and affects the elongation process of transcription by phosphorylating serine-2 at the C-terminal domain of RNA polymerase II. Previous studies have shown that CDK12 plays a role in blastocyst implantation, deletion of CDK12 in a mouse embryo resulted in embryo lethality, but to my knowledge the function of CDK12 in the oocyte has not been investigated. Our main objective was to create a viable mouse model with conditional knockout of CDK12 using Cre-recombinase expressed under the oocyte specific Zona pellucida-3 promoter to study the...
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