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Physiological and pathophysiological role of GCPII in the body
Sedlák, František ; Konvalinka, Jan (advisor) ; Klener, Pavel (referee) ; Smetana, Karel (referee)
Glutamate carboxypeptidase II (GCPII) is a metalloprotease responsible for cleaving the neurotransmitter N-acetyl-aspartyl-glutamate in the central nervous system to N-acetyl aspartate and glutamate. At the same time, in the human small intestine, it facilitates folate absorption by cleaving γ-linked glutamate from folyl-poly-γ-glutamate. In humans, GCPII is also expressed in a number of other organs (e.g., kidney and prostate) and tumors, where its physiological function is unknown. In an attempt to characterize the physiological function of the enzyme, we first characterized the commercially available monoclonal antibodies against GCPII. Further, we developed a fully synthetic replacement based on a hydrophilic polymer with bound GCPII inhibitors. We evaluated the suitability of using a murine biomodel to study GCPII function in vivo. We found the difference in GCPII expression profile in mouse and human. We did not observe GCPII in either the mouse prostate or small intestine. To assess physiological and pathophysiological functions of the enzyme we analyzed a GCPII-deficient mouse model. Apart from the observation of enlarged seminal vesicles in older males, we did not detect any other obvious phenotype. Similarly, we confirmed that GCPII cannot cleave amyloid peptides (Aβ1-40 and Aβ1-42)....
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The effect of intelligent transport systems to increase the safety of inhabitants of villages (Libkov, Rozvadov, Kašperské Hory)
Sedlák, František ; Kocmánková Menšíková, Lucie (advisor) ; Zewdie, Retta (referee)
The aim of the thesis is to determine how Intelligent Traffic Systems (ITS) affects road safety and thereby contributes to increase the safety and quality of life in selected villages (Libkov, Rozvadov, Kašperské Hory) of Pilsen region. The theoretical part contains basic concepts of regional development and policy with a focus on road traffic, quality of life and safety of the population, and also description of devices which can be used as means to calm traffic and improve the safety of the population. Empirical part was done by developing a secondary analysis of data on accidents in road traffic and the impact of ITS on the safety of residents in selected villages. Secondary data analysis of the ITS revealed, that the main causes of drivers accidents in the Czech Republic (from 2014) are considered to be bad driving (62.9%) and speeding (17.5%). Libkov, Rozvadov and Kašperské Hory belong to project "Peaceful Border Areas", which aims to reduce speed on municipal roads and thus increase the safety of population in the villages using installed SYDO Traffic Zeus speed indicators. Thanks to the evaluation of these ITS has been found that in selected villages vehicles slowed down on average by 38.6%, leading to increased road safety and improved quality of life in Libkov, Rozvadov and Kašperské Hory villages.
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