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Behavioral changes in fish after methamphetamine exposure
SLIVONĚ, Miroslav
Illegal drug use has adverse consequences in the global society, but what about other organisms or ecosystem? According to the results of recently published studies, the use of illegal drugs also has a negative effect on organisms in the aquatic environment, where it enters via insufficiently treated wastewater. This diploma thesis is focused on the effect of the illegal drug methamphetamine (METH) on the change in the behaviour of brown trout (Salmo trutta morpha fario) at environmentally relevant concentrations (1000 ng/L). The results of the experiments confirmed the presence of METH and its major metabolite amphetamine (AMFA) in the liver and brain tissue of exposed fish. The changes in fish behaviour suggest that exposure to METH affected the spatial orientation of fish, expressed by left laterality, and the locomotion, represented by higher exploratory activity of exposed fish. However, the effect of METH has not been demonstrated in the presence of fish near the feed site. Based on the results of this laboratory study, the negative impact of METH and its metabolites on individuals, populations, communities, and possibly entire ecosystems was concluded.
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Vliv běžných pesticidů na lidské buňky
SLIVONĚ, Miroslav
The effect of terbuthylazine-2-hydroxy (HTBA) on human cell cultures of HepG2 and MG63 was investigated by method of acute in vitro toxicity tests at concentrations of 0; 0.58; 0.8; and 8 mg/l. The experiment has two parts. In the first part overall biological activity (mortality, specific growth rate, dilation - cytoskeletal activity) was examined using a Nikon Biostation IM-Q microscopic device. The evaluation of the acquired images took place visually and the significant effect of HTBA was not confirmed. In the second part of the experiment, performed on a high-resolution NanoTruth light microscope, data corresponding to a concentration of 8 mg/l HTBA and data from a control experiment without added HTBA tested on HepG2 cell culture were evaluated. The evaluation of the obtained image data was performed mathematically, using the optical flow method. From the resulting values of optical flow, lower intracellular dynamics was found in the exposed sample than in the sample without the presence of HTBA. This suggests a possible negative effect of HTBA on the tested cell culture.
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