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Diurnal changes of Rubisco activity and its content in spinach leaves
Riegerová, Jana ; Vespalcová, Milena (referee) ; Hrstka, Miroslav (advisor)
The graduation theses theme was to determinate changes of initial activity, total activity and Rubisco enzyme content in spinach leaves. Ascertained activities and Rubisco enzyme content were determined from extracts of spinach leaves, which were collected during two distinctively climatic different days. Both activities were determined by spectrometric method and content of enzyme Rubisco by SDS-PAGE method. The findings were compared with findings determined in earlier done works.
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Morphological changes of erythrocytes in selected diseases
RIEGEROVÁ, Jana
This bachelor's thesis deals with the morphology of ecrocytes and their anomalies related to selected diseases. The theoretical part of the thesis is focused on the description of individual ecrocyte morphological abnormalities and the description of selected diseases for which morphological transformations are typical. It also aims at the explanation of their importance for diagnosis and disease progress. The practical part comprises description of blood smears microscope evaluation procedure, evaluation of erytrocyte morphology and assessment of morphological transformations in regard to patients' diagnoses. More than 80 samples were evaluated, all of which were collected between 2nd May 2017 and 25th May 2017 at the Department of Hematology in Písek. Selected blood smears containing morphological anomalies. Samples were classified in nineteen categories according to the diagnoses. Afterwards, morphological transformations were evaluated in each category. Frequency of identified abnormalities was assessed and then also their possible impact on the disease was considered. Among three most frequently detected erytrocyte morphological transformations belong polychromasia in 45 samples, anisocytosis in 43 samples and ovalocytosis in 22 samples. The observed morphological transformations were in some cases typical for the basic diagnosis and treatment, in some cases the detected morphology was untypical for diagnosed disease and informed the physician of another possible health issue. Patients with untypical signs in blood smears were consequently subjected to additional tests, which revealed the etiopathogenesis of such transformations. The results thus demonstrate the importance of blood smear evaluation for making a precise diagnosis.
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Diurnal changes of Rubisco activity and its content in spinach leaves
Riegerová, Jana ; Vespalcová, Milena (referee) ; Hrstka, Miroslav (advisor)
The graduation theses theme was to determinate changes of initial activity, total activity and Rubisco enzyme content in spinach leaves. Ascertained activities and Rubisco enzyme content were determined from extracts of spinach leaves, which were collected during two distinctively climatic different days. Both activities were determined by spectrometric method and content of enzyme Rubisco by SDS-PAGE method. The findings were compared with findings determined in earlier done works.
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