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Evaluation of genetic strains polymorphism of \kur{Beauveria bassiana} based on analyses of using microsatellite markers
PUDIL, Jiří
Microsatellites, or Simple Sequence Repeats (SSRs), are highly polymorphic loci present in nuclear and organellar DNA that consist of repeating units of 1-6 base pairs in length. Microsatellites are very effective molecular markers, which allow us to examine various aspects of organisms. The aim of thesis is to carry out analyzes of microsatellites and describe the genetic relationships between individual strains and monosporic isolates, which were derived from these strains. Usind SSR I would like to asses the degree of genetic polymorphism between \kur{Beauveria bassiana} strains, collected during monitoring in the locality of Černá Hora in Šumava. I am also trying to optimize the method of DNA isolation and method of microsatellites analysis. 44 strains of \kur{Beauveria bassiana} isolated from \kur{Ips typographus} were analyzed in total using four primers. Overall, the results that I obtained show high similarity of evaluated strains, and therefore low level of genetic polymorphism. For further work it would be appropriate to enlarge the area, which will be sampled and include sets of strains that were obtained from different developmental stages of the host, as well as other reference strains of entomopathogeníc fungi collected from other localities with similar conditions. For more detailed study it would be useful to use the analysis of ribosomal DNA. Thus selected and evaluated population is likely to provide a more veritable image of strain diversity in observed area.
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Specifics of Marketing Communication Targeted on Seniors and Unfair Practices Against Them
Nováková, Vendula ; Průša, Přemysl (advisor) ; Pudil, Jiří (referee)
This master thesis "Specifics of Marketing Communication Targeted on Seniors and Unfair Practices Against Them" aims to familiarize the reader with the ways and coercive methods by which traders force seniors to buy overpriced goods or services during the selling events or doorstep sales. Another aim is to identify the reasons that lead elderly to attend sales events, or use the services of door-to-door seller. Next, I will try to make recommendations to public authorities and elderly how to prevent unfair trade practices. The thesis also deals with legal regulations, the demographic situation and development, and manipulation.
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Evaluation of effectiveness of pilot project E.ON Energieonline communication channels
PUDIL, Jiří
The purpose of this study was to increase the understanding of the effectiveness of different forms of direct marketing ? namely direct mail, direct email and social networks advertising. According to the analysis results the direct mail was offered for the real launch of the product. This decision was confirmed by multi-criteria decision method, which also includes different weight of each criterion.
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Financing of short-term needs of the enterprise.
PUDIL, Jiří
The object of the bachelor thesis is to describe structure of the assets in enterprise, focusing on short time assets. Clasify the rules for short-term financing and evaluate possibilities of these needs with their advantages and disadvantages. The experimental part contains system of relative financial indicators and its application in particular enterprise. The Evaluation of financial situation and suggestions for the enterprise were brougt out in the conclusion of the bacheor thesis.
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