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Difference pulse voltametry for detection of metallothionein
Postránecká, Tereza ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
Metallothionein (MT) is a protein with a characteristic amino acid composition which thanks to its thiol groups can bind metal ions and be involved in the homeostasis of these ions in organism. The protein has its significance in carcinogenesis, could be a suitable marker for certain types of tumors. MT has many specific functions in organisms. It can be studied using several techniques. In common use electroanalysis is used as method for determining the concentration of MT. More specifically, this work is focused on differential pulse voltammetry using Brdička reaction.
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Comparison of methods for RGB spectrogram construction of DNA
Postránecká, Tereza ; Provazník, Ivo (referee) ; Kubicová, Vladimíra (advisor)
This thesis discusses about possibilities of construction colour DNA spectrograms and about patterns which can be detected there. Spectrograms as tools of spectral analysis give us a simultaneous view of the local frequency throughout the nucleotide sequence. They are suitable for gene identification or gene regions identification, determination of global character about whole chromosomes and also give us a chance for the discovery of yet unknown regions of potential significance. For purpose of this kind of DNA analysis is possible to use digital signal processing methods. We can apply them on only after conversion of DNA sequence to numerical representation. Selection of correct numerical representation affects how well will be reflected biological features in numerical record which we need for another use in digital signal analysis.
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Removal of pacing spikes from the electrocardiographic signal
Smíšek, Radovan ; Plešinger,, Filip (referee) ; Postránecká, Tereza (advisor)
The goal of this thesis is to detect pacing pulses in ultra high-frequency ECG so as to remove these pacing pulses. It makes evaluation of higher frequency components of QRS complex possible. This evaluation is impossible while pacing pulses are present. Chosen issue is solved using heuristic algorithm. Algorithm uses spacing of signal by line in the area which is not influenced by pacing pulses. Subsequently this line is made longer and using differences between line and signal (or another rules) edges of pacing pulses are detected. The top of the stimulation tip is detected by thresholding envelope of original signal´s first difference. More algorithms are tested in this thesis. Several methods of removing pacing pulses are suggested in thesis. Envelopes of high-frequency components are created. Envelopes are analyzed subsequently and suggested methods of removing pacing pulses are compared on the basis of these analysis. Finally the detection efficiency is evaluated.
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Biventricular pacing optimization by means of the dyssynchrony parameter
Jurák, Pavel ; Leinveber, P. ; Halámek, Josef ; Plešinger, Filip ; Postránecká, T. ; Lipoldová, J. ; Novák, M.
To improve Cardiac Resynchronization Therapy (CRT), different interventricular delay (VVD) settings can be used. However, relatively small VVD induced hemodynamic changes cannot be measured by standard echocardiographic methods. The QRS complex duration (QRSd) is mostly the main criterion. Here we introduce a new dyssynchrony parameter (DYS) that is able to more accurately detect improved electrical synchrony. Methods: 12-lead 5 kHz ECG during 3-10 minute rest period was measured in 46 patients with CRT OFF and CRT ON with VVD 0 ms (CRT0) and -20 ms (CRT20). We detected QRSd and the dyssynchrony parameter DYS as the time difference between 500-1000 Hz averaged envelopes positions in the V1 and V6 leads in the QRS complex region. Results: 32 of 46 patients had a positive CRT response manifested by QRSd shortening and a DYS decrease. 28 of 32 patients had a positive LV pre-excitation effect: additional QRSd shortening of 4.7+/-.9 ms and a DYS decrease of 12.6+/-7.5 ms. The correlation coefficient of QRSd and DYS changes (CRT 0 vs CRT 20) was 0.23 and indicates information diversity. The DYS parameter differs from QRSd and provides a significantly higher response to VVdelay changes (p <; 0.001).
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Difference pulse voltametry for detection of metallothionein
Postránecká, Tereza ; Prášek, Jan (referee) ; Provazník, Ivo (advisor)
Metallothionein (MT) is a protein with a characteristic amino acid composition which thanks to its thiol groups can bind metal ions and be involved in the homeostasis of these ions in organism. The protein has its significance in carcinogenesis, could be a suitable marker for certain types of tumors. MT has many specific functions in organisms. It can be studied using several techniques. In common use electroanalysis is used as method for determining the concentration of MT. More specifically, this work is focused on differential pulse voltammetry using Brdička reaction.
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|
|
Removal of pacing spikes from the electrocardiographic signal
Smíšek, Radovan ; Plešinger,, Filip (referee) ; Postránecká, Tereza (advisor)
The goal of this thesis is to detect pacing pulses in ultra high-frequency ECG so as to remove these pacing pulses. It makes evaluation of higher frequency components of QRS complex possible. This evaluation is impossible while pacing pulses are present. Chosen issue is solved using heuristic algorithm. Algorithm uses spacing of signal by line in the area which is not influenced by pacing pulses. Subsequently this line is made longer and using differences between line and signal (or another rules) edges of pacing pulses are detected. The top of the stimulation tip is detected by thresholding envelope of original signal´s first difference. More algorithms are tested in this thesis. Several methods of removing pacing pulses are suggested in thesis. Envelopes of high-frequency components are created. Envelopes are analyzed subsequently and suggested methods of removing pacing pulses are compared on the basis of these analysis. Finally the detection efficiency is evaluated.
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Comparison of methods for RGB spectrogram construction of DNA
Postránecká, Tereza ; Provazník, Ivo (referee) ; Kubicová, Vladimíra (advisor)
This thesis discusses about possibilities of construction colour DNA spectrograms and about patterns which can be detected there. Spectrograms as tools of spectral analysis give us a simultaneous view of the local frequency throughout the nucleotide sequence. They are suitable for gene identification or gene regions identification, determination of global character about whole chromosomes and also give us a chance for the discovery of yet unknown regions of potential significance. For purpose of this kind of DNA analysis is possible to use digital signal processing methods. We can apply them on only after conversion of DNA sequence to numerical representation. Selection of correct numerical representation affects how well will be reflected biological features in numerical record which we need for another use in digital signal analysis.
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