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Molecular monitoring of residual disease in chronic lymphocytic leukemia
Forsterová, Kristina ; Schwarz, Jiří (advisor) ; Pospíšilová, Šárka (referee) ; Žák, Pavel (referee)
The detection of residual tumor cells in patients with chronic lymphocytic leukemia (CLL) was the goal of this study. Since there is no CLL-specific molecular marker, minimal residual disease detection (MRD) is not simple. The outcome of 29 patients treated with fludarabine, cyclophosphamide and rituximab (FCR) was monitored using PCR with "consensus" primers and comparative PCR. 16 patients subsequently underwent autologous stem cell transplantation (ASCT) which did not significantly prolong the length of molecular remission reached by these patients in comparison to patients treated with FCR only. However, it seems that the length of molecular remission of transplanted patients was not affected by eventual contamination of progenitor cells with leukemic cells while it was affected by the presence of detectable CLL cells in their bone marrow at the time of ASCT. Since "consensus" PCR is not a method sufficiently sensitive for MRD detection in CLL the usefulness of detection of DNA methylation was explored for this purpose. Methylation status of 5 genes (DLC1, SHP1, p15, p16 and lamin A/C) was analyzed in the tested group of patients. DLC1 methylation was detected in the majority of patients so changes in its methylation levels in relation to disease evolution were further analyzed using real time...
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Business Plan – Expansion of Joinery Company
Pospíšilová, Šárka ; Navrátil, Jaroslav (referee) ; Rompotl, Jaroslav (advisor)
Master‘s Thesis deals with the problem of entreprise and it‘s success rate. The aim of accent is analysis of current business in joinery Antonin Hála – HAANT. To provide the analysis are used analysis of internal and occupational environment of the company but also the important financial analysis. All of them are provided for the purpose to identify the gaps and the possibilities of extension of current enterprise. Based on acquired results of analysis is proposed the extension of entrepreneurial activities.
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The effect of tick´s serpin IRS-2 on dendritic cells activated by TLR4 ligand
POSPÍŠILOVÁ, Šárka
IRS-2 is the inhibitor of serine proteases from the Ixodes ricinus tick. My task in this thesis was to find out the effect of the IRS-2 on dendritic cells activated by TLR4 ligand or by Borrelia afzelii. This effect was studied on several levels. I focused on the cytokine production, the expression of costimulatory molecules and cell signaling pathways. The results show that the IRS-2 may inhibit the expression of costimulatory molecules CD-80 a CD-86 on the cell surface, but this finding needs to be confirmed again. The production of cytokines was not affected by the IRS-2. The effect of the IRS-2 on the activity of p38, Erk1/2 nor NF-?B in LPS stimulated cells vas not observed. The fosforylation of STAT 3 in cells activated by the B. afzelii was lowered by the IRS-2.
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Evaluation of Povrchová důlní, s.r.o. Financial Standing
Náhluk, Stanislav ; Pevná, Jana (advisor) ; Pospíšilová, Šárka (referee)
The goal of this thesis is to apply methods of financial analysis at the selected company and assess that way financial situation. In the theoretical section summarizes the findings of financial analysis, its methods and procedures. The practical section assesses past and current financial state of company Povrchová důlní, s.r.o. in the years 2005 to 2009, by using selected methods of financial analysis. In conclusion of practical part is analyzed company compared with the industry in which operates, and is assessed the impact of the economic crisis on economy of company.
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