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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
Struktura a funkce tmRNA u streptomycet
 Bobek, Jan ; Palečková, Petra ; Mikulík, Karel
The functional trans-translation system is important to allow survival of antibiotic producing microorganism under unfavourable conditions. The ssrA genes from S. coelicolor, S. collinus, S. aureofaciens and S. griseus were amplified and subjected to sequence analysis. To investigate the role of tmRNA in streptomycetes we have examined the level of tmRNA during the growth and differentiation under various physiological conditions
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Aktivace translačního systému v průběhu buněčné diferenciace streptomycet
 Bobek, Jan ; Palečková, Petra ; Vohradský, Jiří ; Mikulík, Karel
Synchronously germinating spores of S. granaticolor were used to study the activation of translational system and expression of proteins. Proteomic approach was used to gain more information on expression of proteins during germination on two types of media differing in ability of cells to produce antibiotic granaticin. The requirement of functional trans-translational system for reactivation of translation in S. aureofaciens exposed to unfavourable conditions was examined. Abundance of tmRNA is connected with changes in the rate of translation and with the accumulation of tetracycline. Our data indicate that trans-translation could be involved in self-defence against antibiotics synthesized
Detailed record
Struktura a funkce tmRNA kontrolující translaci u streptomycet
 Bobek, Jan ; Palečková, Petra ; Mikulík, Karel
The functional trans-translation system is important to allow survival of antibiotic producing microorganism under unfavourable conditions. The ssrA genes from S. coelicolor, S. collinus, S. aureofaciens and S. griseus were amplified and subjected to sequence analysis. To investigate the role of tmRNA in streptomycetes we have examined the level of tmRNA during the growth and differentiation under various physiological conditions
Detailed record

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