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Transport mechanisms of secondary metabolites across membranes of plant cells
Kubeš, Jan ; Tůmová, Lenka (advisor) ; Babula, Petr (referee) ; Obložinský, Marek (referee)
Faculty of Pharmacy in Hradec Králové, Charles University Department of pharmacognosy Candidate: Mgr. Jan Kubeš Supervisor: PharmDr. Lenka Tůmová, CSc. Title of Doctoral thesis: Transport mechanisms of secondary metabolites across membranes of plant cells An attention was focused on the metabolism of isoflavones in recent years, including their transport within plants and cultures in vitro. A research is focused on the transport mechanism identification across cell or vacuolar membrane. There are localized different transporters as or the transport of some metabolites can take place through membrane vesicules. The release of five isoflavones (genistin, genistein, biochanin A, daidzein and formononetin) into nutrient medium was observed in suspension culture of Genista tinctoria L and Trifolium pratense L. These cultures were treated with NH4VO3 solutions (1 a 10 M concentration) when isoflavon content in medium was higher after 24 hours. This increased volume of isoflavones was supressed by different transport mechanism inhibitors. The transport of isoflavones in T. pratense was influenced by ABC inhibitors from MRP subfamily, but genistein concentration in medium was lower after treatment with MDR subfamily inhibitors. Brefeldin A, which blocks vesicular transport, also decreased concentration of...
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Transport mechanisms of secondary metabolites across membranes of plant cells
Kubeš, Jan ; Tůmová, Lenka (advisor) ; Babula, Petr (referee) ; Obložinský, Marek (referee)
Faculty of Pharmacy in Hradec Králové, Charles University Department of pharmacognosy Candidate: Mgr. Jan Kubeš Supervisor: PharmDr. Lenka Tůmová, CSc. Title of Doctoral thesis: Transport mechanisms of secondary metabolites across membranes of plant cells An attention was focused on the metabolism of isoflavones in recent years, including their transport within plants and cultures in vitro. A research is focused on the transport mechanism identification across cell or vacuolar membrane. There are localized different transporters as or the transport of some metabolites can take place through membrane vesicules. The release of five isoflavones (genistin, genistein, biochanin A, daidzein and formononetin) into nutrient medium was observed in suspension culture of Genista tinctoria L and Trifolium pratense L. These cultures were treated with NH4VO3 solutions (1 a 10 M concentration) when isoflavon content in medium was higher after 24 hours. This increased volume of isoflavones was supressed by different transport mechanism inhibitors. The transport of isoflavones in T. pratense was influenced by ABC inhibitors from MRP subfamily, but genistein concentration in medium was lower after treatment with MDR subfamily inhibitors. Brefeldin A, which blocks vesicular transport, also decreased concentration of...
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Transport mechanisms of secondary metabolites across membranes of plant cells
Kubeš, Jan ; Tůmová, Lenka (advisor) ; Babula, Petr (referee) ; Obložinský, Marek (referee)
Faculty of Pharmacy in Hradec Králové, Charles University Department of pharmacognosy Candidate: Mgr. Jan Kubeš Supervisor: PharmDr. Lenka Tůmová, CSc. Title of Doctoral thesis: Transport mechanisms of secondary metabolites across membranes of plant cells An attention was focused on the metabolism of isoflavones in recent years, including their transport within plants and cultures in vitro. A research is focused on the transport mechanism identification across cell or vacuolar membrane. There are localized different transporters as or the transport of some metabolites can take place through membrane vesicules. The release of five isoflavones (genistin, genistein, biochanin A, daidzein and formononetin) into nutrient medium was observed in suspension culture of Genista tinctoria L and Trifolium pratense L. These cultures were treated with NH4VO3 solutions (1 a 10 M concentration) when isoflavon content in medium was higher after 24 hours. This increased volume of isoflavones was supressed by different transport mechanism inhibitors. The transport of isoflavones in T. pratense was influenced by ABC inhibitors from MRP subfamily, but genistein concentration in medium was lower after treatment with MDR subfamily inhibitors. Brefeldin A, which blocks vesicular transport, also decreased concentration of...
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