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Biosensors based on modified nanoparticles
Mihajlović, Ana ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This thesis deals with biosensors based on modified semiconductor quantum dots(AIIBIII). This work is divided into three main parts. The first deals with the theory necessary for the use of QDs in bioapplications, there are described methods of synthesis, application and bioconjugation of QDs . In the practical part, GSH-CdTe, CdTe-MPA and TGA-CdTe quantum dots were prepared by direct colloidal synthesis. These quantum dots were then modified by EDC in order to increase affinity to BSA (bovine serumalbumin). For the analysis of samples were used Infinite M200,Tecan and HORIBA Jobin Yvon FluoroLog detector Quanta . The third part is the summary of results and work achievements.
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Synthesis of core/shell quantum dots for diagnostics
Mihajlović, Ana ; Vaculovičová, Markéta (referee) ; Pekárková, Jana (advisor)
This thesis deals with biosensors based on modified semiconductor core/shell quantum dots (QDs) for diagnosis. The work is divided into four main parts. The first one discusses the theory required for the use of QDs in bioaplications, there are described methods of synthesis, modification, application and bioconjugation of QDs. In the experimental part, CdTe/ZnS QDs with core/shell structure were prepared, in which the core was modified by MPA, GSH and TGA. In the next step, these QDs were further modified using CDI, EDC and NHS as mediators in order to increase affinity to BSA (bovine serum albumine) and IgG (imunoglobuline G). Prepared conjugates were characterized by fluorescence spectroscopy (Infinite M200Pro, Tecan) and capillary electrophoresis (Agilent 7100).
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Biosensors based on modified nanoparticles
Mihajlović, Ana ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This thesis deals with biosensors based on modified semiconductor quantum dots(AIIBIII). This work is divided into three main parts. The first deals with the theory necessary for the use of QDs in bioapplications, there are described methods of synthesis, application and bioconjugation of QDs . In the practical part, GSH-CdTe, CdTe-MPA and TGA-CdTe quantum dots were prepared by direct colloidal synthesis. These quantum dots were then modified by EDC in order to increase affinity to BSA (bovine serumalbumin). For the analysis of samples were used Infinite M200,Tecan and HORIBA Jobin Yvon FluoroLog detector Quanta . The third part is the summary of results and work achievements.
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Synthesis of core/shell quantum dots for diagnostics
Mihajlović, Ana ; Vaculovičová, Markéta (referee) ; Pekárková, Jana (advisor)
This thesis deals with biosensors based on modified semiconductor core/shell quantum dots (QDs) for diagnosis. The work is divided into four main parts. The first one discusses the theory required for the use of QDs in bioaplications, there are described methods of synthesis, modification, application and bioconjugation of QDs. In the experimental part, CdTe/ZnS QDs with core/shell structure were prepared, in which the core was modified by MPA, GSH and TGA. In the next step, these QDs were further modified using CDI, EDC and NHS as mediators in order to increase affinity to BSA (bovine serum albumine) and IgG (imunoglobuline G). Prepared conjugates were characterized by fluorescence spectroscopy (Infinite M200Pro, Tecan) and capillary electrophoresis (Agilent 7100).
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