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Human Dental Pulp Stem Cells Cultured in Xenogeneic-Free Supplemented Media
Suchánková Kleplová, Tereza ; Jouklová, Nela (advisor) ; Merglová, Vlasta (referee) ; Bartoňová, Marie (referee)
Human dental pulp stem cells cultured in xenogeneic-free supplemented media Summary Introduction: The topic of the study is the cultivation of dental pulp stem cells (hDPSCs) in a xenogeneic-free culture medium. It is not permissible to use cells upon growing under the influence of xenogeneic (extraneous) substances in human clinical practice. The most frequently used in cultivation of hDPSCs is fetal calf serum (FCS/FBS). Unfortunately, these supplements are widespread in hMSCs cultivation, and all gold standard hMSCs properties were postulated in cells cultivated using these supplements. This raises the basic question if and how xenogeneic blood derivatives affect the properties of cells and their growth characteristics. There are two options for replacing these xenogeneic substances in the culture medium: the so-called serum-free media, or human blood supplements, ideally autologous ones. The conducted research was aimed at identifying the effects of xenogeneic and human blood supplements on basic hDPSCs characteristics that are fundamental to introduce the cell therapy into regular medical practice. Method: By culturing 12 hDPSC lines obtained from adult, deciduous, and natal teeth in 12 different culture media, we investigated the effect of FCS, human blood derivatives, i.e., blood plasma (HP), and...
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Contemporary methods of treatment of injuries and their complications in immature permanent teeth in children
Tzigkounakis, Vasileios ; Merglová, Vlasta (advisor) ; Nováková, Květoslava (referee) ; Gojišová, Eva (referee)
This study deals with the evaluation of endodontic treatment using calcium hydroxide materials in immature permanent teeth that have sustained traumas, especially after severe periodontal tissue injuries. - The aim of this work was: a. to analyze the factors that contribute to occurrence of post-traumatic complications, especially after avulsion injuries in immature permanent teeth , b. to investigate the effectiveness of endodontic therapy of these complications with the use of calcium hydroxide materials in immature permanent teeth, c. to evaluate various calcium hydroxide materials, d. to compare our results with other studies in literature concerning calcium hydroxide properties in cases that it is used as an intra-canal medicament. - The pre-clinical and clinical parts of this thesis are divided in 3 parts: 1. Evaluation of primary schools teachers` knowledge on providing first aid in cases of tooth avulsion injuries: immediate and proper first aid and fast transfer of children to dental practitioner are determining factors for prognosis of the injured tooth and for future occurrence of post-traumatic complications. 300 teachers were asked to fill questionnaires about avulsion of permanent teeth. 69 % of the respondents had never been informed about providing first aid in dental trauma situations, 34 %...
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Children's dental fear as a minority factor in caries prevalence
Vašáková, Jana ; Bradna, Pavel (advisor) ; Merglová, Vlasta (referee) ; Nováková, Květoslava (referee)
Dental caries is a preventable multifactorial infectious disease which results from the overall impact of biological, behavioural and psychosocial factors. The environment of every individual plays a role in its aetiology, too. The content and form of the diet, regularly performed oral hygiene, fluoride intake and continuous dental care act as an unquestionable part in the prevention of dental caries. "Quality of life" or "well-being" is nowadays an emphasized aspect in most of the studies. There is one factor linked to the well-being of a child discussed on a field of paediatric dentistry - the dental fear. The four following studies presented in this dissertation describe the above mentioned topic. The first surveys the dentist's relationship with the child patients and looks for the reasoning why the children were referred to the faculties and specialists. The second pursues the environmental factors which influence the perception of dental environments in pre-schoolers and the fear of even the most common dental procedures. The third performs an evaluation of the dental fear level in a sample of preschool children in the Czech Republic, with correlation to their dental status and geographic location. This study also deals with a possible use of colours as another tool for dental fear evaluation...
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Contemporary methods of treatment of injuries and their complications in immature permanent teeth in children
Tzigkounakis, Vasileios ; Merglová, Vlasta (advisor) ; Nováková, Květoslava (referee) ; Gojišová, Eva (referee)
This study deals with the evaluation of endodontic treatment using calcium hydroxide materials in immature permanent teeth that have sustained traumas, especially after severe periodontal tissue injuries. - The aim of this work was: a. to analyze the factors that contribute to occurrence of post-traumatic complications, especially after avulsion injuries in immature permanent teeth , b. to investigate the effectiveness of endodontic therapy of these complications with the use of calcium hydroxide materials in immature permanent teeth, c. to evaluate various calcium hydroxide materials, d. to compare our results with other studies in literature concerning calcium hydroxide properties in cases that it is used as an intra-canal medicament. - The pre-clinical and clinical parts of this thesis are divided in 3 parts: 1. Evaluation of primary schools teachers` knowledge on providing first aid in cases of tooth avulsion injuries: immediate and proper first aid and fast transfer of children to dental practitioner are determining factors for prognosis of the injured tooth and for future occurrence of post-traumatic complications. 300 teachers were asked to fill questionnaires about avulsion of permanent teeth. 69 % of the respondents had never been informed about providing first aid in dental trauma situations, 34 %...
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