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Vliv transportu auxinu na regulaci vyrůstání kotylárních pupenů hrachu
Macoszek, Ondřej
The diploma thesis was aimed on role of polar auxin transport in regulation of cotyledonary bud outgrowth in pea plants (Pisum sativum L.) in relation to competitive canalization of auxin sources. Decapitation of growth apex, as primary source of auxin, causes release of cotyledonary buds from inhibition. This was followed by increased expression of genes for auxin transport proteins PsPIN1 and PsAUX1 and decrease in PsDRM1 and PsBR1 gene expression that are related to dormant state of plant tissues. In the next phase, different types of exogenous auxin application on decapitated stumps and cotyledonary bud growth dynamic were carried out. Auxin application on decapiteded stump caused inhibition of cotyledonary bud growth, its application on right or left side of the stump inhibits cotyledonary bud on the side of application. If auxin on the both sides of stump was applied, cotyledonary bud growth was not inhibited, on contrary to their separation with a plastic sheet, which resulted in significant growth inhibition of both buds. In case of both-sided application, these sources of auxin prevents each other in canalization. If these two sources of auxin were separated with a plastic sheet, canalization of auxin flow was observed and two independent auxin channels were formed. Analogy of this competition can be expected in the case of cotyledonary buds, as two potential auxin sources.
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Vliv strigolaktonu na větvení kořenů u rostlin huseníčku rolního
Macoszek, Ondřej
In this bachelor study the influence of the concentration of GR24, the synthetic analogue of strigolactone, on the length of the primary root of different ecotypes of Arabidopsis plants was studied. Six ecotypes of A. thaliana were chosen and the experiment was performed at the Gregor Mendel Institute in Vienna in the laboratory of Dr. Wolfgang Busch. The used GR24 concentrations ranged from 12.5 nM to 50 microM. It was known from previous work (Ruyter and Spira 2010, Kol-tai 2011, Vacková 2013) that GR24 influences the root architecture of Arabidopsis thaliana, but the results differed. From the experimental results it is clear that the influence of GR24 on the primary root length depends on the individual plant genotype, but generally it is possible to suggest that low strigolactone concentrations cause inhibition, subsequently higher stimulation of the primary root length and finally concentration above 10 microM are highly inhibitory in all genotypes. Based on the results the concentration of 100 nM was chosen for the subsequent Genome wide association study (GWAS).
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