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Implemenation of the RAD sequencing methods to the population genetic studies of hedgehogs from the genus Erinaceus
Loudová, Miroslava ; Černá Bolfíková, Barbora (advisor) ; Choleva, Lukáš (referee)
Hedgehogs from the genus Erinaceus are an important model organism for studying the postglacial recolonisation of Europe and the processes that take place in the secondary contact zones of their areas of distribution. In this study, five individuals of white-breasted hedgehog (Erinaceus roumanicus), four individuals of western hedgehog (Erinaceus europaeus) and one estimated hybrid were analysed. Geographical distribution of individuals used in the study covers the region of the Central Europe, however in the further research expansion of analsysed individuals will be needed and the whole Palearct should be sampled. The main goal was to implement novel methods in research of hedgehogs, which will enable to map the population-genomic structure of the genus Erinaceus in western Palearct. The method RADSeq (Restriction site associated DNA sequencing) enables to obtain polymorphic markers, e.g., SNPs which we used (Single Nucleotide Polymorphisms) across the genome. In this work it was analyzed 16382 SNPs. Using the binary data which indicates the presence and absence of SNPs for each species, hypotheses raised under classical analyzes of genetic markers from previous studies have not been fully confirmed. In further research it will be necessary to verify possible occurrence of biases connected with...
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Genetic structure of mammalian invasive populations on islands
Loudová, Miroslava ; Černá Bolfíková, Barbora (advisor) ; Hájková, Petra (referee)
Biological invasion is understood as introduction, establishment and expansion of organisms into new, non-native area. This is a natural process that is significantly compounded by human activities and is one of the factors that threaten biological diversity and stability of natural ecosystems. Understanding to the evolution and current status of invasive populations is especially important for planning a project to eradicate invasive species from certain areas to avoid their negative impact on the environment. One option for the research of the invasive populations and their history is the analysis of genetic material. This thesis summarizes the current knowledge on invasive populations of small mammals on the islands, with the focus on genetic studies. Specifically, the study of Norway rats (Rattus norvegicus) on Moturemu island and five archipelagos off the coast of Brittany; black rats (Rattus rattus) on Congo Cay, Madagascar, Guadeloupe Archipelago, Sainte Anne Archipelago and Canary Islands; Pacific rats (Rattus exulans) on Tetiaroa Atoll; house mice (Mus musculus) on Marion Island; house mice (Mus musculus domesticus) on Kerguelen Archipelago; red squirrels (Sciurus vulgaris) on Jersey island, West European hedgehogs (Erinaceus europaeus) in New Zealand; pygmy shrews (Sorex minutus) in Ireland and...
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Implemenation of the RAD sequencing methods to the population genetic studies of hedgehogs from the genus Erinaceus
Loudová, Miroslava ; Černá Bolfíková, Barbora (advisor) ; Choleva, Lukáš (referee)
Hedgehogs from the genus Erinaceus are an important model organism for studying the postglacial recolonisation of Europe and the processes that take place in the secondary contact zones of their areas of distribution. In this study, five individuals of white-breasted hedgehog (Erinaceus roumanicus), four individuals of western hedgehog (Erinaceus europaeus) and one estimated hybrid were analysed. Geographical distribution of individuals used in the study covers the region of the Central Europe, however in the further research expansion of analsysed individuals will be needed and the whole Palearct should be sampled. The main goal was to implement novel methods in research of hedgehogs, which will enable to map the population-genomic structure of the genus Erinaceus in western Palearct. The method RADSeq (Restriction site associated DNA sequencing) enables to obtain polymorphic markers, e.g., SNPs which we used (Single Nucleotide Polymorphisms) across the genome. In this work it was analyzed 16382 SNPs. Using the binary data which indicates the presence and absence of SNPs for each species, hypotheses raised under classical analyzes of genetic markers from previous studies have not been fully confirmed. In further research it will be necessary to verify possible occurrence of biases connected with...
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Genetic structure of mammalian invasive populations on islands
Loudová, Miroslava ; Černá Bolfíková, Barbora (advisor) ; Hájková, Petra (referee)
Biological invasion is understood as introduction, establishment and expansion of organisms into new, non-native area. This is a natural process that is significantly compounded by human activities and is one of the factors that threaten biological diversity and stability of natural ecosystems. Understanding to the evolution and current status of invasive populations is especially important for planning a project to eradicate invasive species from certain areas to avoid their negative impact on the environment. One option for the research of the invasive populations and their history is the analysis of genetic material. This thesis summarizes the current knowledge on invasive populations of small mammals on the islands, with the focus on genetic studies. Specifically, the study of Norway rats (Rattus norvegicus) on Moturemu island and five archipelagos off the coast of Brittany; black rats (Rattus rattus) on Congo Cay, Madagascar, Guadeloupe Archipelago, Sainte Anne Archipelago and Canary Islands; Pacific rats (Rattus exulans) on Tetiaroa Atoll; house mice (Mus musculus) on Marion Island; house mice (Mus musculus domesticus) on Kerguelen Archipelago; red squirrels (Sciurus vulgaris) on Jersey island, West European hedgehogs (Erinaceus europaeus) in New Zealand; pygmy shrews (Sorex minutus) in Ireland and...
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