National Repository of Grey Literature 37 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Study of properties of bent FRP reinforcement
Lipoldová, Marie ; Prokeš,, Jan (referee) ; Bodnárová, Lenka (advisor)
The diploma thesis deals with the study of bent FRP reinforcements with the main focus on the methods of production of bent FRP reinforcement, examples of their application in structures. The work also mentions the effects of aggressive environments on the durability of FRP. In the practical part, a search of the possibilities of testing the properties of bent FRP reinforcement. Subsequently, an experiment is designed and performed to monitor changes in the properties of straight and bent FRP reinforcement exposed to the alkaline environment and water at 20 °C and 40 °C. Finally, the evaluation of changes in mechanical properties and observation of reinforcements using optical and scanning electron microscopy is performed.
Cement Composite for 3D Printing Technology
Lipoldová, Marie ; Hela, Rudolf (referee) ; Bodnárová, Lenka (advisor)
3D printing technology represents a fast-growing industry within many industrial sectors. This bachelor thesis is focused on possibilities of using 3D printing technology in building industry. In the theoretical part there are stated typical technologies and realizations of 3D printing in the field of construction. The thesis also includes a research which is essential for the determination of requirements for cement composites for 3D printing technologies including tests for verification of their properties. The experimental part of the bachelor thesis is focused on testing of various recipes of cementitious composites, especially on the properties of both fresh and hardened composites. Two batches were tested for the suitability of utilisation of waste PET particles.
Factors affecting the protective effect provided by sand fly saliva immunization on the outcome of Leishmania infection
Jelínková, Kristýna ; Kolářová, Iva (advisor) ; Lipoldová, Marie (referee)
ENGLISH VERSION Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania, transmitted by its vector - female sand flies during the process of blood feeding. Preexposure to sand fly saliva prior to Leishmania infection affects the host immune system, leading to possible establishment of protective effect on the disease outcome. This effect is based on the delayed type hypersensitivity reaction to sand fly saliva which has been shown to peak about 48 hours after antigen encounter, leading to the more effective host immune response towards the Leishmania parasites. In this project, we found this protective effect as species-specific, additionally dependent also on the timing of the infection post last immunization. Results of presented experiments showed that infection of BALB/c mice with Leishmania major performed after 48 hours post last Phlebotomus duboscqi sand fly saliva immunization, did not confer protection in comparison to infection performed at one-week after the last exposure. Moreover, the effect of the sand fly saliva pre-exposure was at this shorter timing of infection rather diminished, supported by insignificant differences in the measured parameters compared to the unpre-exposed infected control group. Additionally, immunization with sand fly Sergentomyia...
Genetic regulation of Leishmania infection
Sohrabi, Yahya ; Lipoldová, Marie (advisor) ; Krulová, Magdaléna (referee) ; Kolářová, Iva (referee)
6 Abstract Leishmaniasis is a neglected tropical disease, which belongs to the top health problems because it is endemic in 98 countries in Asia, Africa, the Americas and the Mediterranean region, and is gradually expanding to new areas, including Central Europe and USA. Clinical manifestations of leishmaniasis include a diverse range of forms, ranging from non-lethal cutaneous leishmaniasis to potentially lethal visceral leishmaniasis. Asymptomatic cases are known to exist in endemic areas. Different species of Leishmania induce distinct symptoms, but even the patients infected by the same species develop different symptoms and may respond differently to the treatment. Thus, one of the challenges is to explain the observed variability of leishmaniasis that cannot be attributed to the currently known factors. To find novel regulatory factors of the disease we tested molecules that were shown to play role in other infections and mapped loci controlling parasite load after L. major infection. We also determined genetic control of survival after infection with tick-borne encephalitis virus (TBEV) in order to establish whether there are common elements in response to L. major and TBEV. Interferon-induced GTPases (guanylate-binding proteins, GBPs) play an important role in inflammasome activation and mediate...
Strong Epistasis in Genetics of Leishmaniasis - Identification of Genes and Mechanisms
Krayem, Imtissal ; Lipoldová, Marie (advisor) ; Černá, Marie (referee) ; Krulová, Magdaléna (referee)
Leishmaniasis, a disease caused by Leishmania parasites, ranks as the leading neglected tropical disease in terms of morbidity and mortality. Genotype of the infected organism is an important factor that influences susceptibility to and manifestations of this disease. To study human disease using mouse models, several strains are required, which could collectively exhibit different human pathophysiology. In the current thesis, we investigated the genetic influence on resting levels of immune cells in mice, because these resting levels could influence susceptibility to many of clinical disorders, including infectious diseases; and we performed a systematic review of the role of host genetics and cytokines in Leishmania infections. Moreover, we employed systems genetics to map genes causing susceptibility to leishmaniasis, and to identify additional mechanisms controlling response to Leishmania parasites. Also, we fine mapped the locus Leishmania major response 15 (Lmr15) in order to functionally characterize its role and to identify novel potential candidate genes regulating the response to L. major. Finally, we show a novel role of guanylate binding proteins 2b and 5 in the resistance to L. major. Strain B10.O20 carrying 3.6% of O20-derived genes on the C57BL/10 genetic background, on chromosomes...
Genotype influence on development of infections caused by Trypanosomatidae in mouse
Šíma, Matyáš ; Lipoldová, Marie (advisor) ; Krulová, Magdaléna (referee) ; Kolářová, Iva (referee)
Parasitic protists of genera Trypanosoma and Leishmania are members of Trypanosomatidae family. In our studies, we investigated genetic influence on infections caused by these parasites in a mouse model. These diseases are on genetic level controlled by quantitative trait loci (QTLs), when the resulting phenotype is controlled by set of genes with small individual effect. As a mouse model for mapping of QTLs controlling these infections, we used recombinant congenic strains (RCS). Each RCS carry unique set of 12.5% of the genome from donor parental strain on genetic background of other parental strain. For mapping of QTLs controlling infections caused by Trypanosoma brucei brucei (T. b. brucei) and Leishmania tropica (L. tropica) and eosinophil infiltration into inguinal lymph nodes after Leishmania major (L. major) infection, we used RCS from CcS/Dem series, where STS is donor strain and BALB/cHeA is strain of genetic background. First, it was necessary to find suitable model strains for mapping. In all three studies, we selected RCS, which exceeded range of monitored phenotype parameters in comparison with any other tested RCS or parental strains. Mice of RCS CcS-11 showed shorter survival after T. b. brucei infection and strain CcS-9 exhibited higher eosinophil infiltration after L. major infection. For...

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