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Bloodmeal sources of European sand flies (Diptera: Phlebotominae) and methods of their identification
Kykalová, Barbora ; Dvořák, Vít (advisor) ; Kočišová, Alica (referee)
Leishmaniasis is a dangerous neglected disease with worldwide distribution. It is transmitted exclusively by phlebotomine sand flies. Knowledge of host feeding patterns provides valuable information about the vector/host interaction. Moreover, the identification of host preferences can help to design effective protective measures. Over the years, many methods were adapted for this purpose. Nowadays, serological methods and DNA-based approaches in many variants are the most common ones. Markers of choice for sequencing analyses are mitochondrial genes, especially cytochrome B. Novel methods for host identification in sand flies are based on protein analysis. The method of peptide mass mapping was successfully tested and presented. This thesis presents results of blood meal identification in laboratory-reared female sand flies as well as specimens collected in the field surveys of different Balkan countries. It compares basic identification method, sequencing analysis, based on two different gene markers (cytochrome B, prepronociceptin) and two other DNA based methods, multiplex PCR and RFLP. It also presents use of peptide mass mapping, an inovative method of, mass spektrometry, and compares this approach with traditional DNA-based methods. Key words: Phlebotomus; host identification; DNA-based...
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Identification of bloodmeal sources in phlebotomine sand flies and other bloodfeeding arthropods
Kykalová, Barbora ; Dvořák, Vít (advisor) ; Spitzová, Tatiana (referee)
Hematophagous arthropods that are involved in transmission of many infectious diseases have profound importance in human and veterinary medicine. Bloodmeal identification considerably contributes towards better understanding of vector-borne diseases and vector-host interaction. It reveals epidemiologically significant data on reservoir hosts and degree of anthropophily of studied arthropods. Methods of bloodmeal identification evolved from field observations, serological and DNA-based methods towards protein analyses. Nowadays, the most frequent methods are DNA-based methods but new methods are still being developed. This bachelor thesis summarizes and compares used methods with the primary focus on phlebotomine sand flies, which are involved in transmission of leishmaniases. Key words: sand flies, bloodmeal identification, hematophagous arthropods, analysis
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