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Faktory indukce tvorby hlíz lilku bramboru (Solanum tuberosum L.) v in vitro podmínkách
Kůrková, Jana
This thesis deals with the factors responsible for induction of the formation of tuber potato (Solanum tuberosum L.) in in vitro conditions. The aim was to observe cultivation of the nodal segments of stems on the induction medium with reduced content of inorganic nitrogen 12 umol, 80 g/l sucrose and the addition of 10 mg/l BA for the for-mation of tubers. The frequency of tuberisation was evaluated, as well as morfological changes, size and weight of the tubers. Three groups of explants were established diffe-ring in lenght of cultivation on the induction medium. These were monitored for chan-ges in the content of endogenous ABA in the nodal segments of stem and stolon. Moni-toring of the changes in content of endogenous cytokinin, nitrogen content, production of ethylene, ethane and CO2 was performed as well. Permanent microscopic preparations were prepared to detect transformation of the axillary bud into stolon, resp. tuber. Increased content of ABA during the tuber formation demonstrates its effect on tuberi-zation. Amongs cytokinins, the biggest effect of cytokinins on tuberization has BA, iP and iPR. Conversely, Z and ZR had no influence. Contents of ethylene, ethane,CO2 and nitrogen are related to the lenght of culturing on the induction medium.
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Relationship Between Spirituality and Morality in the Context of Social Work
Kurková, Jana ; Milfait, René (advisor) ; Žáková, Lenka (referee)
This bachelor theses deals with relationship between spirituality and morality in the context of social work. Their connection is greatly reflected in attitudes and behavior of people to themselves as well as to others. The theses provide inspiration and ways of solving problems in the field of social work, for students and graduates of social disciplines, as well as for social, pastoral workers, layperson and for those who are concerned with the lives of others. The aim of this work is to highlight the importance of interconnection of biblical Christian spirituality and morality and their consequences in social work. At the same time it is based on the fact that spirituality and morality are mutually enriching. The work is grounded on biblical message and the relevant documents relating to morality and spirituality, including church documents, Compendium of the Social Doctrine of the Church and ethnical codes.
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Morfogenetické změny v procesu tvorby hlíz lilku bramboru (Solanum tuberosum L.) v in vitro podmínkách
Kůrková, Jana
This work deals with the morphogenetic changes in the process of formation potato tubers (Solanum tuberosum L.) in vitro conditions. The aim was to determine the optimal length of the cultivation the nodal segments of stems on the induction medium with reduced content of inorganic nitrogen 12 micromolar, 80 g/l sucrose and the addition 10 mg/l BA for the formation of tubers. And to confirm the assumption that reduced the amount of inorganic nitrogen in the medium generally leads to a significant induction of tuberization potato (Solanum tuberosum L.). Morphological changes were observed during tuberization and recorded the frequency of tuberization. It was founded the four variants and the control variant was differing length cultivation on the induction medium. It was watched the changes in the content of endogenous ABA in the nodal segment of stem, leaf, stem and tuber during tuberization. It was found that a longer period cultivation on induction medium with a reduced content of inorganic nitrogen in the nitrate form leads to a higher and earlier tuberization of potato (Solanum tuberosum L.). By measuring the content of ABA, it was found that the content of ABA increases with the time during which explants are subjected to stress. Increased content of ABA at the time the tubers illustrates the effect of ABA on the induction of tuberization in potato (Solanum tuberosum L.).
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