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Coaxial and emulsion nanofibers for biomedical use
Korbelová, Gabriela ; Buzgo, Matej (advisor) ; Pavlů, Barbora (referee)
The current treatment of tissue injuries deal with numerous problems, such as limited healing capacity and immune response of the body. In many cases such obstacles does not allow a full regeneration of the injured tissues and the full resumption of its original features. Controlled delivery of growth factors has considerable importance for inducing wound healing for a wide range of defects. Biocompatible and biodegradable nanofibrous carriers prepared by electrospinning represent a universal platform for the delivery of a wide range of substances in regulated manner. The goal of this thesis is to create an overview and assessment of the nanofibrous systems for controlled drug release created by electrospinning with a focus on growth factors delivery. Nanofiber carrier offers a number of advantages, they are able to consistently deliver bioactive substance into the site of injury, maintain bioactivity of the substance and stimulate the adhesion, proliferation and differentiation of target cells. Compared to the classic methods of tissue engineering using tissue grafts with the risk of their immune rejection these funkcionalized biodegradable carriers offers the possibility to create cell-free carrier systems. The work is divided into 4 chapters concerning the methods of preparation of nanofibrous...
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Coaxial nanofibers with incorporated suplements for regulated chondrogenic differentiation
Korbelová, Gabriela ; Rampichová, Michala (advisor) ; Vištejnová, Lucie (referee)
In the field of regenerative medicine, regeneration of cartilage defects (caused either by injury or age-related degeneration) has become a widely discussed topic. Nanofibrous scaffolds provide a suitable environment for cell adhesion, proliferation, differentiation, and also for the local involvement of bioactive substances. Nanofibrous scaffolds mimic the extracellular matrix (ECM) of hyaline cartilage. These scaffolds are seeded with autologous chondrocytes. After having been isolated from the patient, the cells must be cultivated in vitro in order to obtain a sufficient amount of chondrocytes. Scaffolds with cultivated chondrocytes are later implanted back into the pacient. Chondrocytes, however, when grown on a 2D tissue culture plastic rapidly de-differentiate and thus lose the ability to synthesize ECM molecules. The aim of the work was modulation of chondrogenic differentiation medium through finding the ideal concentration of chondrogenic supplements, composed of L-ascorbate-2-phosphate (A2P) and dexamethasone (DEX), in the culture of primary chondrocytes seeded on a nanofibrous polycaprolactone (PCL) scaffold. The effect of different concentrations of the chondrogenic supplements on chondrocyte adhesion to the scaffold and their proliferation and differentiation was studied. The influence...
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Coaxial nanofibers with incorporated supplements for regulated chondrogenic differentiation
Korbelová, Gabriela ; Rampichová, Michala (advisor) ; Vištejnová, Lucie (referee)
In the field of regenerative medicine, regeneration of cartilage defects (caused either by injury or age-related degeneration, such as osteoporosis) has become a widely discussed topic. Nanofibrous scaffolds provide a suitable environment for cell adhesion, proliferation, differentiation, and also local involvement of bioactive substances. Nanofibrous scaffolds mimic the extracellular matrix (ECM) of hyaline cartilage and thus have the potential to treat cartilage defects. The aim of the work was modulation of chondrogenic differentiation medium through finding the ideal concentration of chondrogenic supplements, composed of ascorbate-2- phosphate and dexamethasone, in the culture of primary chondrocytes of pig origin seeded on a nanofibrous polycaprolactone (PCL) scaffold. The effect of different concentrations of the chondrogenic supplements on chondrocyte adhesion to the scaffold and their proliferation and differentiation was studied. Firstly, the influence of each of the supplements alone in the medium was studied, followed by study of effects of their combinations. Then, the supplements were incorporated into the nanofibers and their effect upon their release from the nanofibers was investiaged. The supplements were studied in 21-day experiments. The chondrogenic re- differentiation was best...
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Coaxial and emulsion nanofibers for biomedical use
Korbelová, Gabriela ; Buzgo, Matej (advisor) ; Pavlů, Barbora (referee)
The current treatment of tissue injuries deal with numerous problems, such as limited healing capacity and immune response of the body. In many cases such obstacles does not allow a full regeneration of the injured tissues and the full resumption of its original features. Controlled delivery of growth factors has considerable importance for inducing wound healing for a wide range of defects. Biocompatible and biodegradable nanofibrous carriers prepared by electrospinning represent a universal platform for the delivery of a wide range of substances in regulated manner. The goal of this thesis is to create an overview and assessment of the nanofibrous systems for controlled drug release created by electrospinning with a focus on growth factors delivery. Nanofiber carrier offers a number of advantages, they are able to consistently deliver bioactive substance into the site of injury, maintain bioactivity of the substance and stimulate the adhesion, proliferation and differentiation of target cells. Compared to the classic methods of tissue engineering using tissue grafts with the risk of their immune rejection these funkcionalized biodegradable carriers offers the possibility to create cell-free carrier systems. The work is divided into 4 chapters concerning the methods of preparation of nanofibrous...
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