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Analytical evaluation of selected drugs using UHPLC I
Kalužíková, Barbora ; Kovaříková, Petra (advisor) ; Kučera, Radim (referee)
Barbora Kalužíková Analytical assessment of selected drugs using the UHPLC I Thesis Charles University, Faculty of Pharmacy in Hradec Kralove Study Pharmacy The aim of this work has been to develop an analytic method for the evaluation of sobuzoxane, ICRF-154, and dexrazoxane using UHPLC-UV. Furthermore to verify the linearity of various sobuzoxane concentrations for quantitative evaluation, test of the stability of sobuzoxane in a working solution, and examine sobuzoxane in acid/base stress tests. UHPLC-UV method with gradient elution was developed for the analysis of sobuzoxane, ICRF-154, and dexrazoxane. Analyte separation was achieved using a Zorbax-Aq rapid resolution HT chromatograpjic column (3 mm x 100 mm; 1,8 µm). Methanol and 2 mmol/l ammonium formate acidified to pH 4 with formic acid were used as the mobile phase at following gradient 0 min - 4,5 min 20 % methanol, 4,5 min - 8 min 80 % methanol, 8 min - 11 min 20 % methanol. Analytes were recorded using a UV detector set at a wavelength of 254 nm. Linearity of the method for sobuzoxane was verified in the range (50 µg/ml - 400 µg/ml) using linear regression. The developed method was used to test stability of sobuzoxane in a working solution during analysis and under acid/base stress conditions. The original goal of analyzing one working...
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