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Cultivation and using of herbs at primary school
KŮŽELOVÁ, Kristýna
This diploma thesis is focussed on the cultivation and subsequent use of herbs (medical plants) in teaching at the 1st grade of primary school. The literary part contains information about cultivation and collection of herbs, an overview of selected species and the effects of these medical plants. Part of the diploma thesis is the creation of an educational program for pupils of a selected class on the topic of cultivation and using herbs. The work also deals with a survey at selected primary schools where the current level of cultivation was monitored and the use of herbs in primary schools. The results of the questionnaire are used to create a picture of whether teachers use school gardens or school interiors to grow medical plants or herbs.
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Validation of HPLC evaluatoin of piroxicam in plasma using SPME and precipitation
Kuželová, Kristýna ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Validation of HPLC evaluatoin of piroxicam in plasma using SPME and precipitation Rigorous Thesis Mgr. Kristýna Kuželová Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové The purpose of this thesis was bioanalytical evalution of piroxicam using High Performance Liquid Chromatagraphy (HPLC). Piroxicam was isolated from plasma using SPME and protein precipitacion. Plasma was adjusted to pH 2,5. SPME was composed of 20 minutes sorption on PDMS/DVB fiber and 20 minutes desorption into 200 ul of methanol. In the second method the sample of plasma was precipitated with acetonitril, 30 seconds shaked and then 5 minutes centrifuged at 5 000 G. Then the supernatant was removed and analysed with HPLC. HPLC analysis was on the column with reversed phase C18. The mobile phase consist of water and acetonitrile (60:40 v/v), pH of solution was adjusted to 2,5 using the formic acid. The flow rate was 1 ml/min, the temperature was set at 40řC. The detection was carried out at 333 nm. SPME and protein precipitation were validated according to FDA. Specificity, accuracy, precision, recovery, linearity, limit of detection a quantification and stability were monitored.
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Analytical evaluation of active substances liquid chromatography VI.
Kuželová, Kristýna ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
ANALYTICAL EVALUATION OF ACTIVE SUBSTANCES BY LIQUID CHROMATOGAPHY VI. Diploma Thesis Kristýna Kuželová Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové The method for determination of the piroxicam in rabbit plasma using solid phase microextraction (SPME) and high performance liquid chromatography (HPLC) with UV detection was optimalized. Fiber coated with PDMS/DVB was used for microextraction. Isoxicam was chosen as an internal standard. The sample of plasma was adjusted to pH 2,5. Microextraction was composed of 20 minutes sorption and 20 minutes desorption into 200 μl of methanol. Column with reversed phase C18 was used for separation. A solution of water and acetonitrile (60:40 v/v) was used as the mobile phase. Its pH was adjusted to 2,5 using formic acid. The flow rate was 1 ml/min and temperature on the column was set at 40řC. The detection was carried out at 333 nm. Linearity and selectivity of the method were verified. Detection and quantification limits for piroxicam were also determined.
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Analytical evaluation of active substances liquid chromatography VI.
Kuželová, Kristýna ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
ANALYTICAL EVALUATION OF ACTIVE SUBSTANCES BY LIQUID CHROMATOGAPHY VI. Diploma Thesis Kristýna Kuželová Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové The method for determination of the piroxicam in rabbit plasma using solid phase microextraction (SPME) and high performance liquid chromatography (HPLC) with UV detection was optimalized. Fiber coated with PDMS/DVB was used for microextraction. Isoxicam was chosen as an internal standard. The sample of plasma was adjusted to pH 2,5. Microextraction was composed of 20 minutes sorption and 20 minutes desorption into 200 μl of methanol. Column with reversed phase C18 was used for separation. A solution of water and acetonitrile (60:40 v/v) was used as the mobile phase. Its pH was adjusted to 2,5 using formic acid. The flow rate was 1 ml/min and temperature on the column was set at 40řC. The detection was carried out at 333 nm. Linearity and selectivity of the method were verified. Detection and quantification limits for piroxicam were also determined.
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Validation of HPLC evaluatoin of piroxicam in plasma using SPME and precipitation
Kuželová, Kristýna ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Validation of HPLC evaluatoin of piroxicam in plasma using SPME and precipitation Rigorous Thesis Mgr. Kristýna Kuželová Charles University in Prague, Faculty of Pharmacy in Hradec Kralove, Department of Pharmaceutical Chemistry and Drug Control, Heyrovského 1203, Hradec Králové The purpose of this thesis was bioanalytical evalution of piroxicam using High Performance Liquid Chromatagraphy (HPLC). Piroxicam was isolated from plasma using SPME and protein precipitacion. Plasma was adjusted to pH 2,5. SPME was composed of 20 minutes sorption on PDMS/DVB fiber and 20 minutes desorption into 200 ul of methanol. In the second method the sample of plasma was precipitated with acetonitril, 30 seconds shaked and then 5 minutes centrifuged at 5 000 G. Then the supernatant was removed and analysed with HPLC. HPLC analysis was on the column with reversed phase C18. The mobile phase consist of water and acetonitrile (60:40 v/v), pH of solution was adjusted to 2,5 using the formic acid. The flow rate was 1 ml/min, the temperature was set at 40řC. The detection was carried out at 333 nm. SPME and protein precipitation were validated according to FDA. Specificity, accuracy, precision, recovery, linearity, limit of detection a quantification and stability were monitored.
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