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The role of mitochondrial DNA in reproduction
Svobodová, Mariana ; Daňková, Pavlína (advisor) ; Jelínková, Ladislava (referee)
The bachelor's thesis focuses on the role of mitochondrial DNA (mtDNA) in human reproduction, especially describing the differences in mtDNA dynamics in oogenesis, spermatogenesis and early embryo development. The physiological function of mtDNA is complemented by its pathologies and their effect on fertility and the ability of the embryo to implant successfully. Furthermore, mtDNA is discussed as a possible diagnostic marker in the evaluation of the quality of sperm, eggs and embryos. These findings of these studies are especially significant for assisted reproduction, where the goal is to select the highest quality embryo with the greatest implantation potential. The work also briefly mentions the presence of cell free mtDNA in the mother's blood and its connection with the development of preeclampsia. Key words: mtDNA, mitochondria, reproduction, (in)fertility
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Correlation of morphokinetic parameters of human preimplantation embryos with their chromosomal status
Kosařová, Zuzana ; Jelínková, Ladislava (advisor) ; Knytl, Martin (referee)
A significant amount of human preimplantation embryos is aneuploid. Preimplantation genetic testing of aneuploidies (PGT-A) enables us to examine the number of chromosomes in a few trophectoderm cells biopsied from developing embryos, and only euploid embryos are then recommended to be transferred. Biopsy of trophectoderm cells is an invasive method, PGT is quite expensive, the examination is not performed at all patients. Therefore, non-invasive methods for determining the highest quality embryos are searching. Non- invasive continual monitoring in a time-lapse system is able to observe the morphokinetic parameters of embryonic development, and can select the best developing embryos with a good prognosis for successful implantation. However, there is still the question of whether and how morphokinetic parameters and embryonic ploidy correlate. Most studies on this topic show that a combination of both of these approaches is the best way for selecting the highest quality embryo for transfer.
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